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Histo-morphology of Cutaneous Papillomatosis in Indigenous Cattle

U. Ayman
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

S. K. Das*
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

Background Papillomatosis is a viral disease manifested with benign cutaneous growths (skin epithelium hyperplasia) in different body parts which is encountered in cattle, goat, dog, rabbit, horse, rodent and also in human. The purpose of the present study is to detect the histo-morphological changes of cutaneous papillomatosis in indigenous cattle. Methods Ten (10) random samples from skin lesions of warts were collected from adult indigenous cattle (10 cattle) that were diagnosed as cutaneous papillomatosis by clinical examination at Veterinary Teaching Hospital, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. For gross observations, location of appearance of warts, color and size was taken into consideration. Immediately after collection of sample, they were preserved in Bouin’s fluid followed by dehydration, clearing and infiltration and finally embedded by paraffin wax. Then samples were sectioned using rotatory microtome and stained with Harris’s Haematoxylin and Eosin Y to facilitate microscopic study. Results The results of present study revealed that cutaneous papillomas were mostly located on the head and neck, around eyes, under the chin and perineal region in indigenous cattle. Grossly, warts in cattle are bumpy or cauliflower-like masses of various sizes and numbers that projected from the skin having grey, rough, scaly, and dry surface. When incised, its cut surface has an external layer- keratinized epithelium and an internal core- moist white connective tissue. Histopathological changes indicate hyperplastic features of the affected skin layers of the epidermis. Further, the microscopic analysis also revealed orthokeratotic hyperkeratosis, acanthosis and down word hyperplasia, fibrovascular area, keratohyalin granules and koilocytes at different layers of the affected skin. Conclusion The results of this present study might be useful for the classification and treatment of this common malady based on its histological changes.

Keywords: Papillomatosis, Histo-morphology, Indigenous cattle

Location: Veterinary Teaching Hospital, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Start Date:

End Date:

Program Area: Pest Management

Commodity: Cattle, Diseases

Objectives:

The purpose of the present study is to detect the histo-morphological changes of BPV induced cutaneous wart in indigenous cattle.

Methodology:

Sample collection

Randomly ten (10) wart samples were collected from different sites of body (head, neck, around the eyelid, perineal region) from adult indigenous cattle (10 cattle) chronically affected with cutaneous papillomatosis from different cases at Veterinary Teaching Hospital, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. After recording gross observations, samples were placed in a sterile container with Bouin’s fluid and transported to the laboratory as soon as possible for further investigation.

Histopathology

After proper fixation in Bouin’s fluid, the samples were dehydrated using 70%, 80%, 90%, 100%, 100% and 100% alcohol for 2 hours in each and overnight for the last one followed by clearing using three changes of xylene, 2 hours at each and infiltration using three changes of paraffin, 30 minutes in each. The paraffin-embedded tissues were cut into 5 micron thick section using rotatory microtome and stained with Harris’s Haematoxylin and Eosin Y to facilitate microscopic study.

Source of Information: Bangl. J. Vet. Med. (2019). 17 (1): 47–52 ISSN: 1729-7893 (Print), 2308-0922 (Online)

Article Link (Online Journal): DOI: https://doi.org/10.33109/bjvmjj19fam2

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The common predilection site for cutaneous papillomatosis in indigenous cattle is head, neck, around eye, perineal region and sometimes may spread other parts of the body. The results of this present study might be useful for the diagnosis, classification and both for prophylactic and therapeutic treatment of this common malady based on its histological changes.

Knowledge Management: Journal