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Semen Characteristics of Black Bengal Buck in Relation to Age, Body Weight and Scrotal Circumference

M.R. Islam
Animal Division, National Institute of Biotechnology (NIB), Atomic Energy Research Establishment AERE), EPZ, Savar, Dhaka-1349

S. Afroz
Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh

M.A.M.Y. Khandoker
Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh

Q.S. Akter
Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh

A.H.M.S. Rahman
Reproductive Biotechnology Laboratory, Department of Animal Breeding and Genetics, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted to find out semen quantity and quality in relation to age, body weight and scrotal circumference (SC) of Black Bengal buck, the most important goat breed in Bangladesh. Semen was collected from four bucks by the method of ariificial vagina (AV). After collection semen was evaluated for volume per ejaculate (ml), mass motility (%), individual motility (%), morphology (% normal and abnormal sperm) and concentration (million/cc) in relation to age, body weight and SC. Age, body weight and SC of four different bucks were 34 to 45 months, 20.0 to 33.6 kg and 20.1 to 21.0 cm, respectively. The mean volumes per ejaculate were 0.4±0.03 to 0.5±0.22 ml and mass motility was found 76.0±1.5 to 78.0±1.1%. On the other hand individual motility in terms of progressive, oscillatory and rotatory were 65.7±0.1 to 68.0±1.3%, 5.0±0.6 to 6.1±0.4% and 4.0±0.4% to 5.4±0.3%, respectively. While the (normal and abnormal sperm) and sperm concentration were found to be 89.7±0.35 to 91.2±0.4% and 8.8±0.4 to 10.3±0.4% and 2434.0±52.8 to 2853.0±90.1 (million/ml), respectively.

Keywords: Black Bengal buck, Semen characteristics

Location: In Bangladesh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Goat, Semen

Objectives:

From that standpoint, the present study was therefore undertaken to uncover the effect of age, body weight and scrotal circumference (SC) on different semen characteristics of Black Bengal bucks.

Methodology:

Four adult Black Bengal bucks were selected to produce semen with 80% morphologically normal spermatozoa with satisfactory motility and concentration. The bucks aged between 34-45 months. The body weight and scrotal circumference (SC) of bucks were 20.0 to 33.6 kg and 20.1 to 21.0 cm, respectively. The bucks were reared isolated from does.

The bucks were trained to ejaculate in an artificial vagina (AV) by homosexual mounting. Semen was collected twice a week. After collection, semen was kept at 37°C in water bath. After collecting the semen volume of the ejaculate was measured by reading the graduated tube in milliliter or ml. For observation of mass motility, one drop of semen was mixed with tris buffer and was placed on a clean prewarmed slide (37°C) and covered with a cover slip. The motility was determined by eye estimation on the basis of the movement of spermatozoa at higher magnification (40x). Just after collection the semen was diluted with buffer solution and inclined slowly. When examined under microscope, movement of the sperm was observed with care. Motility was observed by following types of movement: progressive/forward, oscillatory and rotatory movement. In case of normal and abnormal sperm count (%) two drops of buffer was placed on a clean, dry glass slide and one drop of mixed semen was added in buffer. Covering with another slide spread the buffer with semen. Then the slide was removed by pulling parallel gently. The slides were stained with Rose Bengal stain for 4-5 minutes, then it was rinsed with distilled water to remove excess stain. Then the smear was dried in the air. The slide was placed on the stage of microscope and counted in high magnification. A total of 333 sperms were counted for normal and abnormal sperm by using random fields on different parts of the slide. The concentration of spermatozoa (million/cc) was determined by using haemocytometer. The concentration of spermatozoa per ml of semen was expressed as million/ml.

The data generated from this experiment were entered in Microsoft Excel worksheet, organized and processed for further analysis. Descriptive statistics were performed to calculate mean, standard error and percentage. One-way ANOVA test was performed to obtain the difference in sperm motility. Statistical analyses were performed using SPSS (Statistical Package for Social Sciences, SPSS inc. 1999, Microsoft Corporation 1998) windows package.

Source of Information: J. Bangladesh Agril. Univ. 6(1): 65-71, 2008 ISSN 1810-3030

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Semen parameters of Black Bengal bucks varied among different bucks with varying age, body weight and scrotal circumferences although the parameters were in an insignificant level. Whether the differences found among the bucks in relation to age, body weight and scrotal circumferences reflected in the fertility results after Al is yet unknown and needs to be further studied.

Knowledge Management: Journal