Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Anatomical Changes in Chickpea (cicer Arietinum L.) Under Aluminium Stress Condition

Rifat Samad
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Parveen Rashid
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

JL Karmoker
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted to investigate the effect of aluminium (Al) toxicity on the anatomical changes in the root, stem and leaf of chickpea (Cicer arietinum L.) plants grown in sand culture. Toxicity of Al reduced the length of primary root and the number of lateral roots of chickpea than that of the control. Aluminium decreased the size and number of vessels in the root of chickpea. The larger area of sclerenchyma cells was noticed in the stem of the Al-stressed plant. The number of palisade parenchyma was reduced in the leaf of chickpea. Aluminium treatment caused the closure of stomata. An increased number of trichomes in chickpea leaves was also reported due to aluminium.

Keywords: Aluminium toxicity, Anatomy, Chickpea, Sand culture

Location: Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Start Date:

End Date:

Program Area: Risk Management in Agriculture

Commodity: Chickpea, Heavy metal in soil

Objectives:

To assess the influence of Al stress on the anatomical structure of the root, stem, and leaf of chickpea grown in sand culture.

Methodology:

Seeds of chickpea (Cicer arietinum L. var. BARI Chhola-7) were procured from Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur. The experiments were conducted in the net house of the Department of Botany, University of Dhaka, under normal environmental conditions. Plants were grown in sand culture(7) and subjected to half-strength Hoagland solution(8) (pH 4.2) which served as control. Similarly, 150 and 300 µM AlCl₃ solution made in half-strength Hoagland solution (pH 4.2) was applied to each pot containing 7- day-old seedlings which were used as treatments. Control and each treatment had three replications. Later on, half-strength Hoagland solution (pH 4.2) was applied to control plants and 150 and 300 µM AlCl₃ solution (pH 4.2) were applied to respective Al-treated plants every day up to 28 days. The root, stem, and leaf of chickpea plants were collected at 28-day of Al exposure. Freehand sectioning was done and the sections were stained with safranin. Transverse sections of the root, stem, and leaf were studied with a compound microscope. Photographs of sections were taken using a camera (Axiocam ERc 5s) at different magnifications (5X, 10X, and 40X). Leaf stomata and trichome of 28- day-old control and Al-treated plants grown in sand culture were also studied.

Source of Information: Dhaka Univ. J. Biol. Sci. 30(2): 187-196, 2021 (July)

Article Link (Online Journal): DOI: https://doi.org/10.3329/dujbs.v30i2.54645

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Aluminium-induced decreased number and diameter of xylem vessels (Plates 2, 3, 5, 6, 7, 10) would decrease the translocation of ions from the root to the different parts of the plant. So, changes in the root system may affect nutrient uptake, which can lead to nutritional deficiencies in shoots and leaves. Closure of stomata might lower the transpiration rate and also decrease the rate of photosynthesis due to a decrease in CO₂ diffusion through the stomata. To minimize the negative effects of Al toxicity, chickpea plants responded by changing the anatomical structure of the root, stem and leaf and by closing a maximum number of stomata. An increased number of trichomes in the leaf (Plates 12) functioned as a defense mechanism to protect the chickpea plants against Al stress.

Knowledge Management: Journal