Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Isolation of Bioactive Principles and Studies of Antimicrobial, Cytotoxic and Antioxidant Activities of the Stem Bark of Baccaurea Ramiflora (euphorbiaceae)

Manoj Mandal
Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, Bangladesh

Lutful Kabir
Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, Bangladesh

Imran khan
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh

Sheikh Tanzina Haque
Department of Applied Chemistry and Chemical Engineering, University of Dhaka, Dhaka-1000, Bangladesh

Md. Enamul Haque*
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

Phytochemicals are extensively found at different levels in many medicinal plants. Stem bark of Baccaurea ramiflora (common name Latkan) belonging to the family of Euphorbiaceae, has been studied for isolation of its secondary metabolites and evaluation of its bio-activities of the crude extract and fractions with especial emphasis on the antimicrobial, cytotoxic and antioxidant activities. The concentrated chloroform extract was partitioned with petroleum ether, ethyl acetate and methanol and the remaining chloroform crude extract was kept for assessing antimicrobial, cytotoxic and antioxidant activities. After successful separation and purification of the different VLC fractions of the chloroform extract of stem bark B. ramiflora, two secondary metabolites namely BRF5-B1 and BR-F5-B2 were isolated. The structure of this two compounds were elucidated as phytol (1) and betulinic acid (2) by comparing with their 1H NMR spectral data with published values. The disc diffusion method was used to evaluate the antimicrobial activities. The methanol soluble fraction of B. ramiflora stem bark did not show any activity against the testing bacteria. The petroleum soluble fraction (PESF) and crude extract showed mild to moderate antibacterial activities against both gram positive and gram negative bacteria as compared to control antibiotic disc.The ethyl acetate soluble fraction (EASF) revealed mild activity. Staphylococcus aureus, Shigella boydii and Vibrio mimicus showed the maximum sensitivity to the crude extract of B. ramiflora when compared to control (Ciprofloxacin 30μg/disc). The crude extract showed mild activity against certain fungi. In the DPPH assay, different fractions of chloroform extract of B. ramiflora demonstrated significant antioxidant activity. In the brine shrimp lethality bioassay the LC50 value of crude extract of B. ramiflora was 11.09 μg/ml, which demonstrated moderate cytotoxic principles present in the crude extract.

Keywords: Baccaurea ramiflora, Phytochemical, Antibacterial, Antifungal, Cytotoxic, Antioxidant

Location: Norshingdi district of Bangladesh

Start Date:

End Date:

Program Area: Chemical Analysis

Commodity: Fruit

Objectives:

In the present study, isolation of two compounds and antimicrobial, cytotoxic and antioxidant activities of the chloroform extract of the stem bark of B. ramiflora have been discussed.

Methodology:

The stem bark of B. ramifolia was collected from the forest of Norshingdi district of Bangladesh. It was taxonomically identified by a botanist of the University of Dhaka. After proper identification the stem bark was cut into small pieces and dried naturally. The sun-dried stem bark was ground mechanically and extracted in a soxhlet apparatus with chloroform. The extract was then concentrated in vacuo using a Buchi rotavapor. The CHCl3 extract was then fractionated by vacuum liquid chromatography (VLC) over silica gel. Pure compounds were then isolated from different fractions using chromatographic techniques like flash column chromatography and preparative thin-layer chromatography. This provided two compounds, BR-F5-B1 and BR-F5-B2. The in vitro antibacterial and antifungal activities of the crude extract as well as different fractions were determined by disc diffusion methods14. Fourteen bacterial strains and five fungi were collected from the Department of Microbiology and Institution of Nutrition and Food Sciences, University of Dhaka. Brine Shrimp lethality bioassay15 has been used for the assessment of cytotoxicity of the test samples. This bioassay can indicate not only cytotoxicity but also the pharmacological activity of test materials.

Source of Information: Bioresearch Communications Volume 04, Issue 02, July 2018

Article Link (Online Journal): www.bioresearchcommunications.com

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

So, in the bioassay for brine shrimp cytotoxicity, the crude extract of B. ramiflora showed moderate cytotoxicity compared to control which indicats that the compounds present in the sample are biologically active. Test samples showed different mortality at different concentrations and the rate of mortality of brine shrimp was found to be increased with the increasing concentration for each sample. The degree of lethality was directly proportional to the concentration of the extract. The laboratory test also showed that B. ramiflora was also effective against specific bacteria and fungi. So both of these support the traditional uses of this plant in various infectious diseases and also supports the literature findings.

Knowledge Management: Journal