Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Brine Shrimp Toxicity Study of Different Bangladeshi Medicinal Plants

Mohammed Rahmatullah
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh.

Sk. Md. Imrul Sadeak
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh.

Sitesh C. Bachar
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh.

Md. Tozammal Hossain
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh

Abdullah-al-Mamun
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh

Montaha
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh

Nowshin Jahan
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh

Majeedul H. Chowdhury
New York City College of Technology The City University of New York 300 Jay Street, Brooklyn, NY 11201, USA.

Rownak Jahan
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh

Dilruba Nasrin
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh

Mahbubur Rahman
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh

Shahnaz Rahman
Faculty of Life Sciences, University of Development Alternative, Dhanmondi, Dhaka, Bangladesh.

Abstract/ Executive Summary:

Twenty three Bangladeshi medicinal plants used in traditional medicines were evaluated for brine shrimp lethality toxicity. Different solvent extracts of Abroma augusta, Acanthus ilicifolius, Alstonia scholaris, Calotropis procera, Catharanthus roseus, Citrus grandis, Coccinia grandis, Croton tiglium, Cuscuta reflexa, Excoecaria agallocha, Heritiera fomes, Ipomoea aquatica, Lagerstroemia speciosa, Madhuca indica, Musa sapientum, Nypa fruticans, Piper peepuloides, Premna esculenta, Saraca asoca, Sonneratia apetala, Stevia rebaudiana, Syzygium fruticosum, and Trichosanthes dioica were used in the study. Of the 23 plants tested, about 80% were toxic to brine shrimp (LC50 < 30 g/ml). Among the extracts screened, the methanolic extract of Croton tiglium had the highest toxicity to brine shrimp (LC50 = 0.0924 g/ml). The drug vincristine sulfate was considered as the reference standard.

Keywords: Cytotoxicity, Brine shrimp, Bangladesh, Medicinal plants

Location: Various regions of Bangladesh

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants, Brine shrimp

Objectives:

The objective of the present study was to evaluate the cytotoxic potential (LC50) of extracts of plant parts of the above-mentioned plants through the brine shrimp lethality bioassay. The brine shrimp (Artemia salina) bioassay is a useful tool for the isolation of bioactive compounds from plant extracts (Sam, T.W., 1993) The method is often used because it is simple, inexpensive and low amount of materials are sufficient to perform the assays on a micro scale.

Methodology:

Plant materials The plant samples were collected from various regions of Bangladesh based on their ethnomedicinal uses. To determine whether a plant has any ethnomedicinal use, surveys were conducted among folk and tribal medicinal practitioners as described before. All plants were identified at the Bangladesh National Herbarium at Dhaka where voucher specimens were also deposited. The various plant parts were cut into small pieces, air-dried under shade and grounded using a laboratory mill and blender.

Extraction A known amount of dried powdered sample (usually 100g) was extracted by maceration with methanol, acetone, or chloroform at a ratio of plant material to solvent of 1:5. Collected extracts were then filtered and concentrated in vacuo at 40^o C, 50^o C, and 30^o C for methanol, acetone, and chloroform, respectively. The solvent-free extracts were kept refrigerated at 4^o C and used for cytotoxicity assays.

Toxicity testing against brine shrimp Hatching shrimp. Brine shrimp eggs, Artemia salina leach were hatched in artificial seawater prepared by dissolving 38g of sea salt in 1L of distilled water. The pH of the solution was adjusted to 8.5. After 48h incubation at room temperature (26-30^o C) under constant aeration, the larvae (nauplii) were attracted to one side of the vessel with a light source and collected with a pipette. Nauplii were separated from eggs by aliquoting them three times in small beakers containing seawater. Brine shrimp assay. The bioactivity of the extracts was monitored by the brine shrimp lethality test (Meyer, B.N., 1982). Samples were dissolved in dimethylsulfoxide (DMSO) and diluted with artificial sea salt water so that final DMSO concentration did not exceed 0.05%. 50 ml of 2000 mg of the plant extract was placed in one sample tube and a two-fold dilution carried out down the column of sample tubes. The last sample tube was left with sea salt water and DMSO only to serve as the drug-free control. The total volume was adjusted to 5 ml with sea salt water. 100 ml of suspension of nauplii containing 10 larvae was added into each tube and incubated for 24h. The tubes were then examined under a magnifying glass and the number of dead nauplii in each tube counted. Experiments were conducted with control (vehicle treated), and different concentrations of the test substances in a set of three tubes per dose. Vincristine sulfate was used as a positive control in all experiments.

Statistical analysis Lethality assays were evaluated by Finney computer statistical program to determine the LC50 values and 95% confidence intervals. All data were expressed as mean ± SEM.

Source of Information: Advances in Natural and Applied Sciences, 4(2): 163-173, 2010 ISSN 1995-0748

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In the present study, DMSO was used as the solvent and as negative control. This is in accordance with previous report that brine shrimp nauplii can tolerate up to 11% of DMSO (Sam, T.W., 1993). Further studies are being conducted to isolate and purify the bioactive constituents for further evaluation in human cell line cultures for cytotoxic effects.

Knowledge Management: Journal