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Research Detail

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Dipak Kumar Paul
Department of Applied Nutrition and Food Science, Kushtia Islamic University, Kushtia, Bangladesh

'Ranajit Kumar Shaha
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh

Seventeen different common citrus fruits have been analyzed for their content of nutrients: carbohydrate, protein, lipids, vitamins, 13-carotene, thiamine, riboflavin, ascorbic acid, important macro and micro minerals such as sodium, potassium, calcium, magnesium, phosphorous, iron, zinc and copper. Carbohydrate, protein and fats in citrus fruits varied from 4.60-8.50, 5.80-7.90 and 2.50-9.50 g, respectively. The content of 13-carotene, thiamine, riboflavin and ascorbic acid varied different amounts in citrus fruits. The highest contents of [3-Carotene fruits are orange and tomato. Among the analyzed citrus fruits pineapple content the maximum amount of thiamine (0.20 mg 100-1g) and wood apple content maximum riboflavin (0.15 mg 1 g). Amla (Indian gooseberry) fruits contents the highest ascorbic acid 600 mg 100—' g of fresh edible parts of fruits. Lemon contained the highest amount of calcium. The highest amount of magnesium was found in Black berry (49.80 mg 100—' g of edible portion of the fruits). Sodium present in different citrus fruits ranged from 1.0 to 28 mg 100—' g. Wood apple contained the highest amount of phosphorus (98.90 mg 100—' g) among all citrus fruits. Tomato contained the highest amount of Potassium (275 mg 100—' g). The iron content in different fruits ranged from 0.10 to 38 mg 100—' g. Zinc present in fruits ranged between 0.18 to 0.48 mg 100—i g. Copper content in different fruits analyzed ranged from 0.1 to 0.68 mg 100—' g of fruits.

  Citrus fruits, Vitamins, Essential nutrients, Macro, Micro elements
  Northern Region of Bangladesh
  
  
  Quality and Nutrition
  Citrus

The  present  study  describes  the nutritive values  by  analyzing  quantitatively of carbohydrate, protein, lipid, vitamins and minerals in some common citrus fruits in the northern region of Bangladesh.

 

Collection of fruits and sample preparation: Seventeen different  common  citrus  fruits  were  collected  from different markets of Kushtia and Raj shahi city. Following collection, the whole fruits were cleaned and washed with distilled water. Weight of each fruit was recorded and then packed in a polyethylene bag, store at -20°C until analysis.  Edible portion of the  fruits  were  separated carefully and used for all analytical investigation. All the analyses  were  done  in triplicate  and the  results  are representing on a fresh weight basis.

Analytical  procedures:  The  edible portions  of fruits (4.0 g) were taken in a crucible and the moisture content was determined by weight loss of the sample on drying at 105°C for 6 h. Using N x 6.25 calculated protein content from  total  nitrogen  after  determination  of the  total nitrogen by semi-micro Kjeldhal methodrl. Lipid was determined by AOAC official method 922.06. Crude fiber was determined by the method of ICONERP1. The sample was charred and ashes to a constant weight, the residue being quantified as ash by AOAC official method 942.05. 

The nitrogen free extracts (NFE) were considered as total carbohydrate and  was calculated by the  following equation: Carbohydrate  (g 100-1g) = 1 00-(moisture+protein+lipid+fiber+ash) g 100—' g. Thiamine  and Riboflavin were estimated by the methods[9-111.  Ascorbic acid (Vitamin-C)  content was estimated by   2,4-di. nitro-phenylhydrazine method 121, P-carotene was estimated by the method of Holdem.

For the estimation of minerals, 5.0 g of sample was wet ashed using a mixture of 18 M sulphuric acids, 12 M perchloric acid and 16 M nitric acid [0.5: 1.0: 0.5 v/v/v][141. After proper dilution, the concentration of Fe, Zn, Cu, Ca and Mg were determined by measuring atomic absorption, while  sodium  and  potassium were   determined  by  measuring  atomic  emission according  to Milner and Whiteside [151 by using  an  atomic  absorbance  spectrophotometer (Pyr Unicam SP-9). An appropriate dilution  was  done  with 0.5%  lanthanum (w/w)  to overcome  ionic interference  during the  estimation of calcium and magnesium. Estimation of phosphorous was done calorimetrically using the method of Fiske and Subbarow.

Statistical analysis: The data are expressed as the mean and standard deviation.  The  difference of the means values using one-way analysis of variance following by Duncan's multiple range tests (DIVERT) at the level of p<0.05. n = 3.

 

  Pakistan Journal of Biological Sciences 7 (2): 238-242, 2004 ISSN 1028-8880
  
Funding Source:
1.   Budget:  
  

The highest Potassium contents citrus fruits are Tomato (275 mg 100-1 g), Grapes blue variety (249   mg  100-1 g) and pineapple (228 mg 100-1 g), respectively in this study. Fruits are very poor sources of iron, zinc and copper are found in this study. Mango green contains the highest amount of iron (4.5 mg 100-1 g), then jambu and tomato (1.0 mg 100-1g). Similarly zinc contents citrus fruits are orange (0.48 mg) then  litchi (0.32 mg), amla (0.30 mg), Mango green (0.30 mg) 100-1 g of fresh edible parts of citrus fruits. The highest amount of copper contents citrus fruits are star apple (0.68 mg), lemon (0.26  mg)  and   mango green (0.24 mg), respectively 100-1 g of fruits. Some of the citrus fruits analyzed values are very close to that reported by Begum et al.[191. Evidences are accumulating that fruits are not good sources of micronutrients. But the amount we found in  our studies is satisfactory. The  data  presented provide most needed information about citrus  fruits, which will assist nutritionists in the assessment of diet-health relationships in our country.

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