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Investigation of Cytotoxic Potential of Ethanolic Extract of Citrus Limetta Fruit Peel, Paederia Foetida Leaves and Methanolic Extract of Cuscuta Reflexa.

Mohammed Aktar Sayeed
Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh.

Mohammad Mamun Ur Rashid
Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh.

Md. Rifahul Abrar Taiseer
Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh.

Abstract/ Executive Summary:

In this study, the comparative cytotoxic potential of Citrus limetta, Paederia foetida leaves Cuscuta reflexa plant were investigated. The Brine shrimp lethality bioassay was used to evaluate cytotoxicity. These extracts showed remarkable cytotoxic activity, LC50 value of these extracts were 74.814 μg/ml, 42.57μg/ml, 24.013μg/ml respectively compared to vincristine sulphate, a reference drug, LC50 0.52 μg /ml. These leaves extracts of have excellent cytotoxic potential and comparative study is demonastrated here with figures and tables. Among all three extracts Cuscuta reflexa has more cytotoxic potential than both.

Keywords: Citrus limetta, Paederia foetida, Cuscuta reflexa, Cytotoxic Potential , Vincristine Sulphate, LC50, EECL, EEPF, MECR.

Location: Department of Botany, University of Chittagong, Chittagong-4331, Bangladesh.

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

To the comparative cytotoxic potential of Citrus limetta, Paederia foetida leaves Cuscuta reflexa plant are investigated.

Methodology:

The plant Citrus limetta was collected from the local market of Chittagong City, and Paederia foetida and Cuscuta reflexa were collected from the Chittagong Hill Tracts and all samples were authenticated by Dr. Shaikh Bokhtear Uddin, Associate Professor, Department of Botany, University of Chittagong, Chittagong-4331, Bangladesh. The plants Citrus limetta’s fruit peels and Paederia foetida’s leaves were collected, dried and extracted with ethanol and the whole plant of Cuscutta reflexa was dried and extracted with methanol. Then the ethanolic extract of Citrus limetta (EECL) and Paederia foetida (EEPF) and methanolic extract of Cuscuta reflexa (MECR) were used for brine shrimp lethality bioassay to investigate the cytotoxic potential. Brine shrimp lethality bioassay is widely used in bioassay for the bioactive compounds. Here simple zoological organism (Artemia salina) was used as a convenient monitor for the screening. The eggs of the brine shrimp, Artemia salina, were collected from an aquarium shop (Dhaka, Bangladesh) and hatched in artificial seawater (3.8% NaCl solution) for 48 hr to mature shrimp called nauplii. The brine shrimp lethality bioassay was performed to predict the cytotoxic activity of the Citrus limetta, Paederia foetida Leaves, Cuscuta reflexa. For experiment, The test samples (extract) were prepared by dissolving them in DMSO (not more than 50 μl in 5 ml solution) plus seawater (3.8% NaCl in water) to attain concentrations 90.91 µg/ml, 74.07 µg/ml, 56.60 µg/ml, 38.46 µg/ml, 19.60 µg/ml and 9.09 μg/ml, 7.40 μg/ml, 5.60 μg/ml, 3.85 μg/ml, 1.97 μg/ml. A vial containing 50μl DMSO diluted to 5ml was used as a control. Standard Vincristine sulphate was used as a positive control then matured shrimps were applied to each of all experimental vials and control vial. After 24 hrs, the vials were inspected using a magnifying glass and the number of survived naupili in each vial was counted. The mortality endpoint of this bioassay was defined as the absence of control forward motion during 30s observation. From this data, the percent of the lethality of the brine shrimp naupili for each concentration and control was calculated. An approximate linear correlation was observed when the logarithm of concentration versus percentage of mortality ^[8] was plotted on the graph paper and the values of LC50 were calculated Using Microsoft excel 2007.

Source of Information: Journal of Medicinal Plants Studies Year : 2013, Volume : 1, Issue : 1

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Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

These leaves extracts of have excellent cytotoxic potential and a comparative study is demonstrated here with figures and tables. Among all three extracts Cuscuta reflexa has more cytotoxic potential than both.

Knowledge Management: Journal