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Responses of Barley Genotypes for Salt Tolerance Under in Vitro Culture Condition

MR Mallik
Department of Agricultural Botany, Patuakhali Science and Technology University, Patuakhali, Bangladesh.

AJ Swarna
Department of Agricultural Botany, Patuakhali Science and Technology University, Patuakhali, Bangladesh.

A Mobaswera
Department of Soil Science, Patuakhali Science and Technology University, Patuakhali, Bangladesh.

ABMMM Khan
Department of Agricultural Botany, Patuakhali Science and Technology University, Patuakhali, Bangladesh.

MHK Howlader
Department of Agricultural Botany, Patuakhali Science and Technology University, Patuakhali, Bangladesh.

Abstract/ Executive Summary:

An experiment was undertaken at Agricultural Botany Laboratory, Patuakhali Science and Technology, to screen out salt-tolerant barley (Hordeum vulgare L.) genotypes. The evaluation was done based on germination percentage, shoot length, root length, and shoot: root ratio. Five barley genotypes including BARI barley 2, BARI barley 3, BARI barley 4, BARI barley 5, and BARI barley 7 were used in the screening program. The barley genotypes were grown in eight levels of NaCl solution like 0(Control), 20, 40, 60, 80, 100, and 120 mM. The present study shows that salinity reduced the germination percentage of barley and the extent of reduction increased with the increase in the concentration of salinity in the growth medium. Shoot dry weight (1.40 mg), root dry weight (0.21 mg), shoot moisture content (80.0%), root elongation rate (1.81), vigor index (21.30), speed of germination (7.91), and root length (21.67 cm) were obtained from the variety BARI barley 4 with 80 mMNaCL (V3T4). Based on the growth attributes the genotypes BARI barley 7 and BARI barley 4 were found tolerant to salt stress as they have grown up to 60 mM and 80 mMNaCl solutions respectively. The genotypes BARI barley 4 was therefore selected to be grown in the south coastal saline soil of Bangladesh.

Keywords: Salinity, Barley, Genotypes, Salt tolerance, In vitro culture

Location: Department of Agricultural Botany, Patuakhali Science and Technology University, Dumki, Patuakhali

Start Date: 00-00-2018

End Date: 00-00-2018

Program Area: Variety and Species

Commodity: Barley, Soil salinity

Objectives:

To identify salt-tolerant varieties and/or genotypes and to develop saline soil management strategies.

Methodology:

The experiment was conducted at the laboratory of the Department of Agricultural Botany, Patuakhali Science and Technology University, Dumki, Patuakhali during the period of November 2018. The experimental field is located at 22.37⁰ N latitude and 89.10⁰ E longitude at Ganges Tidal Floodplains and falls under Agro-ecological Zone “AEZ 13”. The study locations also lie under Ganges Tidal Floodplain AEZ (AEZ No. 13) in the coastal non-saline zone of Bangladesh. This region occupies an extensive area of tidal floodplain land in the southwest of the country. The area lies at 0.9 to 2.1 meters above mean sea level. Treatments: Nine different salinity concentrations were used in the experiment. Each concentration was considered as an experimental treatment. The different salinity concentrations were obtained by dissolving the required amount of crude salt (collected from a seashore) in distilled water. Before weighing, salts were oven-dried to remove moisture. The control (0Mm NaCl) was maintained using distilled water only. The experiment was designed following 2 factors completely randomized design (CRD). The treatments were as follows. Experimental setup: The experiment was carried out in Petri dishes of 12 cm diameter. A thin layer of cotton was set at the bottom of the Petri dishes. Twenty-five seeds were placed on a cotton bed in a circle pattern. Ten milliliters of treatment solutions of different salinity concentrations were poured in each Petri dish to immerse the seeds partially for ensuring proper aeration. Three replications were maintained for each salinity concentration. The Petri dishes were placed on a table in the laboratory. The seeds were allowed to germinate at room temperature (25±2°C). The required amount of distilled water was added to each Petri dish every day to maintain the same level of the water as on the initial date. Seeds were considered germinated when radicles measured 2 mm in size. A partial view of the experiment is shown in plate-1. Observation and statistical analysis: The number of seeds germinated was recorded starting from 2 days after sowing (DAS) to 12DAS. The results obtained in each day were converted into percentages. Data recorded on crop characters were subjected to statistical analysis through the computer-based statistical program Mstat-C (Michigan State University, East Lansing, Ml, USA) following the basic principles, as outlined by Gomez and Gomez (1984). Significant effects of treatments were determined by analysis of variance (ANOVA) and treatment means were compared at a 5% level of significance by Duncan's Multiple Range Test (DMRT).

Source of Information: Progressive Agriculture 32 (1): 1-9, 2021

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In conclusion, from the above observation of the present study, it may be concluded that seed germination and growth of barley varied according to the change in NaCl levels. The increase of salt stress decreased all studied parameters on germination and seedling growth. Salinity reduced the germination percentage of barley and the extent of reduction increased with the increase in the concentration of salinity in the growth medium. NaCl up to 80 mM could be identified as safe for BARI barley 4 for seed germination. Up to 60 mM NaCl concentration salinity might be considered for seedling growth of BARI barley 7. At higher concentrations like 120 mM NaCl, the root was more affected than the shoot.

Knowledge Management: Journal