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Induced Breeding of Fresh Water Menoda Catfish (hemibagrus Menoda) Using Ovupin Hormone

N Islam
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh, Bangladesh

MF Islam
Department of Fisheries, Bangamata Sheikh Fojilatunnesa Mujib Science and Technology University, Melandah, Jamalpur, Bangladesh

MS Islam
Department of Fisheries, Bangamata Sheikh Fojilatunnesa Mujib Science and Technology University, Melandah, Jamalpur, Bangladesh

MI Miah
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh, Bangladesh

Abstract/ Executive Summary:

An experiment on induced breeding of menoda catfish, Hemibagrus menoda was conducted during the period from May 2017 to July 2018 by using commercial Ovupin hormone (GnRHa, Gonadotropin-Releasing Hormone Analogues) to attain its optimum dose. The study consists of three treatments, each with three replications. The objective of the experiment was to find out the effective dose for induced breeding. The breeding parameters were determined in terms of ovulation, fertilization, hatching, and survival rate. A total of 36 males and 18 females lived broodfish were kept in the ratio of 2:1 for breeding purposes. The commercial Ovupin hormone was injected at the doses of 4 (T₁), 6 (T₂), 7 (T₃) mL Ovupin·kg^-1body weight for female and 1.5(T₁), 2 (T₂), 3(T₃) mL Ovupin·kg^-1 body weight for male in a triplicate replication. In this study, it was found that treatment T₂ demonstrated the best spawning performance for both male and female and were statistically significant (p<0.05). The investigation indicated that the dose of Ovupin hormone 6mL Ovupin·kg^-1body weight for females and 2 mL Ovupin·kg^-1 body weight for males determines the highest spawning performance of menoda catfish which should be recommended for high-quality eggs and larvae.

Keywords: Hemibagrus menoda; Induced breeding; Menoda, Catfish; Ovupin. Hormone

Location: Field Laboratory Complex of Fisheries Faculty in Bangladesh Agricultural University, Mymensingh

Start Date: 00-05-2017

End Date: 00-07-2018

Program Area: Variety and Species

Commodity: Cat fish, Hormone

Objectives:

To estimate the effective dose of hormone for the induced breeding of the species.

Methodology:

The research activities were carried out in the Field Laboratory Complex of Fisheries Faculty in Bangladesh Agricultural University, Mymensingh from May 2017 to July 2018. The brood fishes of Gang magur, Hemibagrus menoda fish were collected from Jhanjail point-1, Kongsho River of the Netrokona district in May 2017. They were reared in earthen rectangular ponds of the Field Laboratory complex. During the rearing period, SIS (small indigenous species of fish) and protein and vitamin-E enriched feed were fed to the fish at a rate of 3.5-5 % body weight. Fertilization, manuring, and liming were performed regularly whenever necessary. The average weight of the males and females were 354±42 g and 738±67g respectively. Males were relatively smaller, elongated, and slender in shape than females. The gravid males and females were selected based on the following criteria: a) males have swollen and reddish urogenital papillae. b) females have rounded and protruding abdomen which is soft when touched with fingers and swollen genital opening sometimes reddish in color. The selected broods were shifted to the circular cemented tank with continuous water showering for about five hours prior to administering hormones. During conditioning, males and females were kept off-feed. For the induced breeding purposes, Gonadotropin-Releasing Hormone analogs (GnRHa) commercially known as Ovupin (100 mg Domperidone + 0.2 mg S-GnRHa) were used in this study. Ovupin hormone vial was bought from a reputed shop in Mymensingh town. This experiment was carried out using commercial Ovupin hormone consisting of three treatments- T₁, T₂, and T_3, each with three replications. A total of 54 lived broodfish were taken and kept at a ratio of 2 male: 1 female in the tanks. Semi artificial (or induced natural) propagation method was adopted which involved synchronized spawning in breeding hapa whereby the injected brood fish (male and female spawners) were placed into a breeding hapa fixed in circular and rectangular tanks (dimensions: 1.22´2.74´0.37m). Double hapa (upper and lower) were used. After ovulation and fertilization, the upper hapa was removed along with the spent spawners while the fertilized eggs settled in the lower hapa which served as a shelter during the incubation period. Some larvae also hatched in the hapa. The weights of selected brood fish and the amount of needed Ovupin hormone solution were measured. Then a disposable graduated syringe of 5 mL was used for injecting the hormone to brood fish. The hormone solution was administered intramuscularly to the female (4, 6, 7 mL Ovupin·kg^-1body weight) and male (1.5, 2, 3 mL Ovupin·kg^-1body weight) between the dorsal fin and lateral line maintaining about 45°angular position with the body. The injected fish were then kept into breeding hapas placed in tanks for synchronized spawning at the ratio of 2:1 (temperature 26°C; pH 7.5; dissolved oxygen 6.5 ppm) and their reproductive behavior was closely monitored. It was observed that the males were in ceaseless pursuance of the females though most often they remained together very closely under the shower. After 24 h of injection, all of the fish were found ovulated. Ovulation and fertilization occurred in the spawning hapa. After ovulation, the upper hapa was removed along with the spent spawners leaving the fertilized eggs. Approximately 100 eggs were placed in plastic bowls to determine fertilization rate with three replications of each and water flow was ensured using porous PVC pipe and outlet facility. Fertilized eggs were identified using a magnifying glass and then counted with the help of a soft thin brush. The larvae were separated from unhatched eggs by siphoning with a 1.5mm rubber hose. Then the larvae were closely observed to see the time of yolk sac absorption for the first feeding of larvae. Aerators were used through the systems for aeration. After completion of hatching, about 6 h post-hatching; the number of larvae/hatchlings in each bowl was counted by siphoning them out. For the determination of survival rate, 300 hatchlings were randomly collected from each replication under a particular treatment and stocked in the trays for 10 days (without feeding). During the experimentation, all other conditions were maintained the same. The number of total live larvae in the tray was counted on 10^th day of the experiment for calculation of survival rate. The temperature, dissolved oxygen (DO), and pH value of water were ranged between 26 °C to 29°C, 5.5 to 6.5 ppm, and 6.7 to 7.5 respectively under different treatments. The data were subjected to a one-way analysis of variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT) at a significance level of p<0.05. The computer software SPSS version 20 was used for statistical analysis.

Source of Information: J. biodivers. conserv. bioresour. manag. 6(2), 2020

Article Link (Online Journal): DOI: https://doi.org/10.3329/jbcbm.v6i2.55242

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The findings of the study will be helpful in commercial hatcheries as it is cost savings. It enables to produce the high-quality seeds by dint of proper application of Ovupin hormone dosages in menoda catfish.

Knowledge Management: Journal