Experimental sites and period The experiments were conducted in the laboratory laboratory of Plant Pathology Department, Bangladesh Jute Research Institute (BJRI), Manik Mia Avenue, Dhaka; Seed Pathology Center, Bangladesh Agricultural.
University (BAU), Mymensingh, Oil Seed Division, Bangladesh Agricultural Research Institute (BARI). The experiments were conducted during the period 15 January 2010 to 5 March 2012. Varieties used Seed of O-9897 was used. 110 kg O-9897 seeds collected from one farmer in Monirampur, Jessore. Containers used For this experiment nine different types of storage containers were used, viz. T1= Tin pot, T2= Plastic pot, T3= Poly bag having 25 µm thickness, T4= Gunny bag, T5= Gunny bag lined with polythene, T6= Earthen pot, T7 = Cloth bag, T8= Brown paper and T9= IRRI Poly bag (Super Grain bag II Z) having 78 µm thickness.
Moisture content of seeds Seeds having two level of moisture were used, viz. i) Recommended moisture content (9.5%) as per reference of Khandakar and Bradbeer (1983) and Bangladesh Gazette (2010). ii) Farmers’ condition.
Bio-chemical analysis (protein and oil %) Procedure of protein analysis Total soluble protein estimation Seed extracts were prepared according to Kalpana and Rao (1997) and the total soluble proteins were estimated according to Lowry et al. (1951) as described below.
Reagents used i) 0.2 M phosphate buffer solution of pH 7.5: a) K2HPO4 (17.418 g) was dissolved in distilled water and was made the volume up to 500 ml with distilled water and b) KH2PO4 (13.609 g) was dissolved in distilled water and was made the volume up to 500 ml with distilled water. Then solution (b) was slowly added to 500 ml of solution (a) until the pH reach to 7.5. ii) Lowry solution: Reagent (a) NaOH (4 g) + Na2CO3 (20 g) dissolved in distilled water and was made up to 1 L with distilled water. Reagent (b) CuSO4 (0.25 g) + Na-K tertarate (0.5 g) dissolved in distilled water and was made the volume 50 ml with distilled water. Then by dissolving 1 ml reagent (a) into 50 ml reagent (b), the solution was kept 1 day in ambient temperature. Discard the solution, and the Lowry solution was prepared. iii) Phenolfolin reagent: Phenolfolin solution was made by dissolving 1 ml phenolfolin to 1 ml distilled water.
Procedure Jute seed (100 mg) was grounded with 5 ml of phosphate buffer using a pestle and mortar and centrifuged. The precipitates were washed twice with 5 ml of phosphate buffer, and made up to volumes of 20 ml with distilled water. This extract solution was used to estimate protein content. 0.5 ml extract was taken in a test tube and 2.5 ml Lowry solution was added, and incubated for 20 minutes. Then 0.25 ml phenolfolin reagent was added and shaken by hand. After developing colour, the optical density was measured within 20 minutes at 660 nm wave length by a double beam spectrophotometer (Model: HITACHI 200-20, Japan), and the total soluble protein was estimated as mg g-1 fresh weight by using the standard curve.
Procedure of oil analysis (Soxhlet extraction) The method described by Akbar et. al. (2009) was used with slight modification. The seed kernels (3g) were grounded using a mechanical method and defatted in a soxhlet apparatus. The extraction was carried out by using three different solvents such as hexane, isopropanol, and petroleum ether. The process continued for 6 hours. Solvent was removed by vacuum evaporation and exposure to heat in a drying oven at 50°C. The amount of oil recovered was calculated as percentage of total oil present in jute seed kernels. Each extraction was run in triplicate and the final value is the average of all.
Statistical analysis Data were analysed statistically and treatments effects were compared by Duncan’s Multiple Range Test (DMRT) (Gomez and Gomez, 1984).