A pot experiment was conducted from November 2018 to April 2019 at the field laboratory of Sylhet Agricultural University, Sylhet, Bangladesh (24089’N, 91088'E). The area is located in the north-eastern part of the country and belongs to Agro-Ecological Zone-20 (AEZ-20) named Eastern Surma-Kushiyara Floodplain and is characterized by a sub-tropical climate. During the experimental period, the area's average maximum and a minimum temperature range from 25.0 to 30.8°C and from 12.1 to 23.3°C, respectively. The amount of rainfall was maximum in October 2019 and minimum during December 2019 and January 2020. Relative humidity varied between 64 and 82% throughout the study period and was highest in October 2019 (82%). Four sugar beet genotypes viz., HI-0044 (G1), HI-0473 (G2), KWS-Allanya (G3), and KWS-Serendara (G4) were collected from the Bangladesh Sugar crop Research Institute (BSRI) and used in the experiment. Salinity stress was introduced using laboratory-grade NaCl. Four level of salinity viz., 0 mM NaCl (S0), 100 mM NaCl(S1), 200 mM NaCl(S2) and 300 mM NaCl(S3) were applied in this experiment. Salinity stress treatment was applied at 35 DAS. Recommended doses of fertilizer for sugar beet were used to grow the plant in all salinity levels. The recommended dose of inorganic fertilizer for this region is 120-30-100-12-3.5-1.2 kg ha-1 for N-P-K-S-Zn-B, respectively. 30% of N, P, and K were supplied from the compost. The experiment was laid out following Completely Randomized Design (CRD) with four replications. Thus, the total number of pots used in the experiment was 64 and the individual pot size was 24 cm (diameter) × 32 cm (depth). Two-third portions of the pots were filled with 8 kg of thoroughly mixed soil. The soil was sandy loam having pH 6.8, which was adjusted by using CaCO3. Fertilizers were applied as basal dose during final soil preparation. After liming, the soil was mixed thoroughly with compost, one-third of Urea and whole Triple superphosphate (TSP), Muriate of Potash (MoP), Gypsum, Zinc Sulphate, and Boric acid. The rest of the Urea was applied in two splits at 60 and 90 days after sowing (DAS). Two seeds were sown in each pot at a three cm soil depth from the surface. During the full crop growing period, care was taken to avoid moisture stress and control weeds, pests, and diseases when necessary in the field condition. Therefore the growing conditions would be considered near potential. The first irrigation was done just after seed sowing and the subsequent irrigation was done four times at 45, 70, 95, and 120 DAS. At the earlier stage, excess water was drained out to avoid water logging conditions due to heavy rain. One extra sugar beet seedling was removed to keep one plant per pot (if both the seeds were germinated) and one seedling was transplanted (if both the seeds failed to germinate) within 13 DAS. Sugar beet is susceptible to weeds at an earlier stage until the sugar beet leaves provide shade over the ground. Hence, weeds were removed from the pot manually at 15, 30, 45, and 60 DAS. Dithane M 45 at the rate of 2.2 kg ha-1 and Score 250 EC 0.5 ml L-1 of water were applied at 15-day intervals from 30 DAS by hand sprayer to control damping-off and sclerotium root rot diseases. For controlling cut warm, tobacco caterpillar, and army warm, Durshban was applied at the rate of 2.5ml L-1 of water at 15 days intervals from 30 DAS by hand sprayer. The data were collected on different growth and yield contributing characters viz. number of leaves (no. per plant), leaf chlorophyll content (relative unit), shoot length (cm), shoot dry weight (g), beet length (cm) beet girth (cm), beet dry weight (g) and total dry matter (g). Data on plant growth, yield contributing attributes, and yield were recorded by following the tropical sugar beet production technology guidelines in Bangladesh. A total number of leaves plant-1 were counted from each pot at 165 DAS. The length of the shoot (the petiole and leaf blade) (cm) of each plant was measured from the base to the top of the leaf by a measuring scale. Leaf chlorophyll content was continuously measured after introducing salinity treatment. Leaf chlorophyll content was measured using portable Soil Plant Analysis Development (SPAD) meter at five different points of five fully expanded leaves between 11:00 am to 12:00 pm. A total number of leaves plant-1 were counted in every pot at 165 DAS. Shoot (from the base to the tip of the plant) length (cm) of every plant was measured by a measuring scale. The beetroot length (cm) and girth (cm) of every pot were measured using a measuring scale and slide calipers, respectively. For beetroot girth, three measurements were taken at the basal, middle, and top part of the beetroot and the average was recorded. The shoot of each plant was separated to determine the fresh weight (g plant-1). The beetroot of each plant was harvested, cleaned to determine the fresh weight of beetroot (g plant-1). The shoot of each plant was separated, sun-dried for several days, and then oven-dried till constant weight at 70°C to determine the dry weight (g plant-1). The beetroot of each plant was harvested, cleaned, cut into small pieces, and sun-dried for several days. After that, oven-dried to till constant weight at 70°C to determine the dry weight of beetroot (g plant-1) Leaf chlorophyll content among different genotypes and salinity levels along 14 days of measurements were analyzed through repeated measure ANOVA. Variation among the genotypes and salinity treatments were analyzed through two-way ANOVA. In case of significant effect, means were separated through a post hoc test (using LSD value). Values were reported as significant at p-values <0.05. All the analysis was performed in R.