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Immunogenicity of Salmonella Pullorum Killed Vaccine in Selected Breeder Flock

Md. Mostafizur Rahman
Upazilla Livestock Office, Bahubal, Habiganj, Bangladesh

Sumana Al Mazid
Upazilla Land Office, Bahubal, Habiganj, Bangladesh

Md. Kamrul Hasan
Eskayef Bangladesh Limited, Bangladesh

Zohirul Islam Rony
Department of Biochemistry and Food Analysis, Patuakhali Science and Technology University, Patuakhali-8602, Bangladesh

Md. Mansurul Amin
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Tanvir Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The present study was carried out to study the immunogenicity of a commercially available formalin-killed alum-precipitated Salmonella pullorum vaccine manufactured by the Livestock and Poultry Vaccine Research and Production Centre (LPVRPC) at the Department of Microbiology and Hygiene, BAU, Mymensingh. vaccination was carried out on Shaver brown chicken (parent stock) of the Phenix Poultry Ltd. Located at Bhaluka, Mymensingh. Birds belonging to group A (n=11,000) were used for vaccination, while birds of group B (n=10) was used as an unvaccinated control group. Each bird was vaccinated intramuscularly at 65 days of age with a subsequent booster dose after 35 days of primary vaccination. Following primary vaccination, the mean value of Passive Hemagglutinating (PHA) antibody titers of vaccinated birds, at 15, 30 and 35 days were 116.00±12.00, 96.00±8.26, and 88.00±11.71 respectively. While, the subsequences mean value of PHA antibody titers of vaccinated birds at 15 and 30 days following the booster vaccination were 128.00±10.12 and 108.00±7.66, respectively. The mean PHA antibody titer in chickens of unvaccinated control birds were ≤ 4.25±0.51. The findings demonstrated that the level of PHA titer after primary vaccination decreased immediately; however, after booster vaccination, the level of titer of PHA antibody increased and sustained. The present study indicated that the S. pullorum killed vaccine prepared by LPVRPC; significantly (p<0.01)) increased the level of PHA antibody in vaccinated chickens compared to unvaccinated control as determined by the PHA test.

Keywords: Salmonella pullorum, Killed vaccine, Immunogenicity, PHA antibody titers, On-farm study, Shaver Brown Chicken.

Location: Livestock and Poultry Vaccine Research and Production Centre (LPVRPC) at the Department of Microbiology and Hygiene, BAU, Mymensingh.

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

In the present study, the immunogenicity of the formalin-killed alum-precipitated S. pullorum vaccine produced by LPVRPC at BAU was studied in Shaver Brown chicken.

Methodology:

The vaccine used in the study was a formalin-killed alum-precipitated S. pullorum-killed vaccine. Vaccination was carried out (on a farm study) intramuscularly in Shaver Brown chicken (Parent stock) (n=11,000) in the Phenix Poultry Ltd. Located at Bhaluka, Mymensingh; following manufacturer’s instructions, 0.5 ml (0.625×1010 CFU) each and these birds were designated as Group A. Unvaccinated birds (n=10) were considered as control and designated as Group B (n=10). Primary vaccination was done at the age of 65 days to all groups of birds except the control group. The booster vaccine was administered to the birds at the age of 100 days. Collection of sera were scheduled as prior to primary vaccination, 15 days after primary vaccination, 30 days after primary vaccination, prior to booster vaccination, 15 days after booster vaccination, and 30 days after booster vaccination. About 1.5- 2 ml of blood samples were collected aseptically from the right jugular vein of each bird using a 5 ml disposable plastic syringe. Collection of sera was accomplished according to Heddleston and Reisinger. Finally, the sera were collected into sterilized glass vials with a rubber cap and stored at -20° C until use. Immunogenicity of the vaccine was investigated by determining the production of antibodies in the vaccinated chicken by PHA test as the method described by Akter et al., A repeated measure ANOVA was performed for significant differences in PHA titers of different ages. P-value at ≤ 0.05 was considered statistically significant. Paired t-test was initiated to locate significant differences between mean PHA titers. Package software SPSS 12.00 version was used to analyze all the data.

Source of Information: International Journal of Natural and Social Sciences, 2016, 3(1): 01-04

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Based on the results of the present work, it could be concluded that the S. pullorum killed vaccine prepared at LPVRPC, Department of Microbiology and Hygiene, BAU, Mymensingh induced a significantly high level of antibody in chickens as determined by PHA test conducted in an on-farm study.

Knowledge Management: Journal