2.1 Experimental site The research was conducted at the pulses research lab and pulses research field in Regional Agricultural Research Station, Jashore, Bangladesh during the rabi season of 2019-2020. The experimental site is located on 23°18' latitude and 89°18' longitude with an elevation of 19 m from sea level (Kobir et al., 2020). The location is under the Agro-Ecological Zone (AEZ11) namely ‘High Ganges River Floodplain’ (BBS, 2019).
2.2.1 Collection of working samples One high-yielding variety BARI Chola-10 was used in this experiment. The variety was collected from Regional Agricultural Research Station, Jashore. Five fungicides namely Provax 200 WP (Carboxin +Thiram), Secure 600 WG (Fenamidone + Mancozeb), Rovral 50 WP (Iprodione), Bavistin 50 WP (Carbendazim) and Captan 50 WP were collected from local market of Jashore. 2.2.2 Preparation of working sample Six conical flasks of 250 ml were filled with 100 g seed of BARI Chola-10. After the addition of adequate water in each conical flask, it was kept overnight for imbibition. Then 250 mg of each fungicide along with 100 g seeds was taken separately in 250 ml conical flasks. With the addition of water drop by drop flasks were shaken manually for 10-15 minutes for proper coating of the fungicides. 2.2.3 Experimental treatment and design The experiment was conducted in a completely randomized design with three replications where seeds were treated with five different fungicidal groups namely, Provax 200 WP (Carboxin +Thiram), Secure 600 WG (Fenamidone + Mancozeb), Rovral 50 WP (Iprodione), Bavistin 50 WP (Carbendazim), Captan 50 WP along with control were applied as treatments. 2.2.4 Germination and seedling vigor test in petri dish Twenty-five seeds were randomly selected from each treatment for each replication. The seeds were placed on water-soaked three folds blotter paper in glass petri dishes. Two petri dishes were taken for each replication for placement of the seeds. Out of the two petri dishes twelve seeds were plated in one and another thirteen seeds were plated in another dish at an equal distance. The seeds were incubated at 25±2ºC.
2.3.2 Climate The climate of the experimental site is subtropical monsoon in nature with adequate rainfall during May to October and a dearth rainfall during November to April. The yearly average rainfall is about 1600 mm and in addition, about 90% of the total rainfall occurs from May to October which is uneven and unpredictable in nature. The monthly average temperature ranges from 20°C in January to 35°C in April. Weather information regarding precipitation, temperature, relative humidity at the experimental site during the period of study has been presented.
2.3.3 Planting material BARI Chola-10 was used as planting material in this experiment. The planting material was collected from pulses seed cold storage of Regional Agricultural Research Station, Jashore. BARI Chola-10 is an erect type variety that is tolerant to drought, heat and botrytis gray mold disease. It usually completes its life cycle in between 112-121 days and the average grain yield is 1800-2030 kg ha-1 (Azad et al., 2019).
2.3.4 Experimental treatment and design In the research field the experiment was conducted in a randomized completely block design with three replications where there were six fungicidal groups as a treatment viz., Provax 200 WP (Carboxin +Thiram), Secure 600 WG (Fenamidone + Mancozeb), Rovral 50 WP (Iprodione), Bavistin 50 WP (Carbendazim), Captan 50 WP and control. Thus, the total number of plots was 18. Five different fungicides @2.5 g kg-1 seeds of BARI Chola-10 were taken in five different pots. After that sterilized water was added in these pots drop by drop which was thoroughly mixed with seeds and fungicides. Then seeds were kept for several minutes to become dry until the fungicides did not clot with the seeds.
2.3.5 Crop husbandry At first the land was ploughed four times by a tractor-drawn cultivator. Then treated seeds were sown @35 kg ha-1 where plant to plant distance of 15 cm and line to line distance of 30 cm was maintained. Fertilizers were applied @20:40:20:10 NPKS kg ha-1 respectively. All the fertilizers were applied as basal during final land preparation. Two times weeding was done 35 days after sowing and 68 days after sowing. Crops were harvested at the proper maturity stage. After harvesting crops were dried in sun and after that dried crops were threshed by power tiller drawn thresher.