Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effects of Application on Growth, Yield and Biochemical Components of BRRI Dhan-55 (oryza Sativa L.)

NASRIN AKHTER DOEL
Department of Botany, Jagannath University, Dhaka-1100, Bangladesh

AMM GOLAM ADAM*
Department of Botany, Jagannath University, Dhaka-1100, Bangladesh

FARHANA ISLAM KHAN
Department of Botany, Jagannath University, Dhaka-1100, Bangladesh

HASNA HENA BEGUM
Department of Botany, Jagannath University, Dhaka-1100, Bangladesh

Abstract/ Executive Summary:

Effects of TIBA (10, 25, 50 ppm) and Rhizobium inoculation on growth, yield and biochemical components of BRRI Dhan-55 were investigated. Results showed that application of 10 ppm TIBA produced tallest plant, higher number of tillers and leaves per plant although statistically identical to control. The Rhizobium and TIBA treatments had mostly retarding effects on dry weight of leaves, shoots and roots where the lowest values was obtained from Rhizobium application. Leaf area ratio was positively affected by TIBA and Rhizobium treatments except due to 10 ppm where, significantly maximum value was noted from Rhizobium treatment. Specific leaf weight, relative growth rate and net assimilation rate were negatively responded following all treatments and the least value was also recorded from Rhizobium treatment in each cases. Yield attributes and yield of BRRI Dhan-55 were both positively and negatively influenced by Rhizobium and TIBA treatments. The highest harvest index was found in Rhizobium treated plants. The only increase in yield per plant due to 10 ppm TIBA was 2.48% over the control but statistically similar to control. Results showed that Rhizobium inoculation had showed lowest value in protein content of leaves and seeds. Application of TIBA treatments had significant positive effects on protein content of leaves at flowering stage. However, the influence was rather negative at both tillering and grain filling stages. The 25 ppm TIBA resulted significantly maximum protein content of seeds followed by 10 ppm.

Keywords: Oryza sativa L., TIBA, Rhizobium, Growth, Yield, Biochemical components

Location: At the botanical garden of the Department of Botany, Jagannath University, Dhaka

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Rice, Bio fertilizer

Objectives:

In Bangladesh, very few experiments have been done with rice using Rhizobium inoculation (Biswas et al. 2000, Hussain et al. 2009). Therefore, an attempt was taken to evaluate the responses of TIBA and Rhizobium inoculation on the growth, yield and biochemical components of rice, var. BRRI Dhan-55.

Methodology:

A pot experiment was conducted at the botanical garden of the Department of Botany, Jagannath University, Dhaka. A high yielding transplanting popular Aush variety, BRRI Dhan-55 which was released in 2011 was used in this investigation. The experiment was laid out in RCBD with five replications for each treatment. Seeds of BRRI Dhan-55 were collected from Bangladesh Rice Research Institute (BRRI), Gazipur whereas, Rhizobium strain (Rhizobium BARI Rca 259) was collected. The experiment consisted of five treatments viz. control (without Rhizobium sp. and TIBA, seed inoculated with Rhizobium sp., foliar application of 10, 25 and 50 ppm TIBA. Seeds were surface sterilized with Captan 200 before sowing to avoid fungal infection and were kept in darkness for 48 hrs for germination. In case of Rhizobium inoculation each seed was inoculated directly with 1 ml of bacterial culture (108 CFU/ml) grown in nutrient broth. After inoculation the inoculated seeds were kept for 2 hrs. Seeds were sown in the seed bed and seedlings were transplanted to experimental pots (25×30 cm) at 4-leaf stage at the age 25 days after sowing. Each pot was filled with 9.0 kg air dried soil. Three hills were placed in each pot, each hill containing one seedling. Cultural practices viz. thinning, irrigation and weeding and fertilizer application were done following Hand Book of Agricultural Technology and Fertilizer Recommendation Guide (2012) respectively. Cow dung was also mixed with soil uniformly during pot preparation. Foliar application of TIBA was done in sunny early morning at 30 days after transplanting. Growth parameters viz. plant height, number of tillers and leaves per plant, dry weight of leaves, shoot and root per plant, leaf area ratio, specific leaf weight, relative growth rate and net assimilation rate and all yield parameters were recorded at harvest. Whereas, Leaf area ratio, specific leaf weight, relative growth rate and net assimilation were calculated using classical growth analysis methods. Pigment and protein contents of fresh leaves were determined at three different stages viz. tillering, flowering and grain filling stages following standard procedures . Protein content of fresh seeds was also determined after harvest. Plants were harvested at the age of 135 days. Ten plants from each treatment were harvested separately to record data on different parameters. Data were analyzed statistically and treatment means were compared by LSD test at 5% level of significance.

Source of Information: Bangladesh J. Bot. 50(4): 1181-1189, 2021 (December)

Article Link (Online Journal): DOI: https://doi.org/10.3329/bjb.v50i4.57087

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Results showed that TIBA treatments had significant positive effects on protein content of leaves at flowering stage. However, the influence was rather negative at both tillering and grain filling stages. Foliar application of TIBA had affirmative response on protein content of leaves at tillering stage and negative effects at flowering and grain filling stages. Results also showed that at harvest, significantly highest protein content of seeds (18.25 mg/g) was obtained from 25 ppm followed by 10 ppm TIBA treatment (13.00 mg/g). The least protein content of seeds (5.05 mg/g) was observed from Rhizobium treated plants which was statistically identical to 50 ppm TIBA but significantly lower than rest of the treatments. The present findings are also in agreement with the findings of Munira (2015).

Knowledge Management: Journal