Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Microbiological Quality Analysis of Fresh Vended Fruit Juices and Water Sold in Roadside Stalls in Dhaka Metropolis by Mpn Method

Mahamuda Akther Eva
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Shawda Shafiq Shreya
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Tasnia Ahmed*
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Abstract/ Executive Summary:

Quality of drinking water and juice is very important because if the quality deteriorates by the contamination of faecally originated microorganisms, it may cause serious diarrhea associated problems leading to death. In overpopulated countries like Bangladesh, this is a common scenario to experience diarrheal diseases due to drinking non-potable water as well as contaminated fresh juices. Present study was conducted to determine the quality of drinking water and juice by detection of indicator bacteria Escherichia coli by MPN (Most Probable Number) method which was performed by three consecutive steps including presumptive test, confirmed test and completed test. Other gram negative bacteria were also identified by biochemical methods. The indicator bacterium Escherichia coli was detected in two water samples out of 15 samples and one juice sample out of fifteen samples respectively during the MPN test method. Other Gram negative bacteria found in both water and juice samples included Klebsiella spp., Alcaligenes spp., Pseudomonas spp. and Proteus spp. The quality of drinking water and juice was found to be good in Bangladesh but proper hygiene should be maintained more strictly to avoid the contamination by other gram negative bacteria which are also capable of causing disease.

Keywords: Fruit juice; Drinking water; Most Probable Number (MPN); Quality; Portability

Location: Dhaka Metropolis city, Bangladesh

Start Date:

End Date:

Program Area: Quality and Nutrition

Commodity: Fruit juices, Water

Objectives:

Present study was designed to determine the quality of drinking water and fruit juices available in roadside tea stalls in Dhaka Metropolis. Fifteen water samples and fifteen fresh fruit juice samples were collected randomly from different areas of Dhaka city for microbiological quality analysis by most probable number (MPN) technique.

Methodology:

Study area and sample processing. Fifteen fruit juice and fifteen water samples were collected from different areas of Dhaka city. After aseptically collection of samples, the samples were transferred immediately to the laboratory for microbial quality analysis by following the procedures proposed by American Public Health Association.

MPN test procedure Presumptive test. Presumptive test is the primary presumption for the presence of Gram negative coliform bacteria in the samples which can be detected by the appearance of gas in the Derhum tubes placed inside the lactose fermentation a broth of 1x concentration) is required for each sample under analysis. Each test tube contained 10 ml of fermentation broth and inoculated with the juice and water samples individually in a sequential order of 10 ml in five of each 2X lactose fermentation broth, 1ml in five of each 1x lactose fermentation broth and finally 0.1 ml in five of each 10 ml 1X lactose fermentation broth. All the test tubes were incubated with half circled screw caps at 37oC for 48 hours. By this procedure all of the 15 samples were subjected to the presumptive test individually.

Confirmed test. Positive samples indicated by the production of gas in the Durham tubes placed inside the lactose fermentation broth were selected for the confirmed test procedures to determine whether the indicator bacteria of fecal origin Escherichia coli was present or not. One loop full sample from the positive test tubes was inoculated on EMB by streaking and incubated at 37^oC for 24 hours and then observed for the production of green metallic sheen. EMB media was used as differentiate media to detect and separate Escherichia coli from other Gram negative coliform bacteria by the production of green metallic sheen in the media. The presence of green metallic sheen in EMB confirms the presence the indicator bacteria E. coli.

Completed test From the green metallic sheen showing streaking line produced by E. coli on EMB media was inoculated into LFB 1X media containing Durham tube to re-confirm the positive lactose fermentation by the detection of gas. From the same place of EMB media, E. coli inoculum was taken to observe microscopically for their Gram reactions. This was the final stage of the MPN method where we can decide that whether the juice under analysis was potable or not. Finally, the standard biochemical tests were performed to confirm the identification of isolates other than Escherichia coli found on EMB agar plates for all of the 15 of fruit juice samples by the standard biochemical tests.

Identification of other bacteria found on EMB. To determine the presence of other gram-negative bacteria, the inoculum from the growth on EMB media were subjected to biochemical identification.

Source of Information: Stamford Journal of Microbiology, 2017. Vol. 7, Issue 1, p. 1-6 ISSN: 2074-5346 (Print); 2408-8846 (Online)

Article Link (Online Journal): DOI: https://doi.org/10.3329/sjm.v7i1.40062

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The overall quality of fresh fruit juices and water is good because only one juice and two water sample was contaminated with Escherichia coli suggesting of faecal contamination. Others were potable according to the absence of the indicator bacteria. The people are becoming more aware about sanitation and hygiene and the quality of processing non-alcoholic beverage items has been increased. But it is needed to be more careful on using water which must be properly purified, and sanitation process should be prioritized more to reduce the number of other contaminating bacteria.

Knowledge Management: Journal