Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Microbiological Analysis and Detection of Anti-bacterial Activity of Centella Asiatica and Aloe Vera Samples Collected from Different Areas of Dhaka City, Bangladesh

Susmita Bhowmik
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Runa Akter Chowdhury
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Md. Aftab Uddin*
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Abstract/ Executive Summary:

The present study was performed to detect the presence of contaminating microorganisms in two commonly available herbal samples (Centella asiatica and Aloe vera) collected from different areas of Dhaka city, Bangladesh and to assess their antibacterial activity. Out of twenty samples (having ten samples of each categories) studied; the range of total viable bacterial count was approximately 103 to 108 cfu/g. Presence of Staphylococcus aureus was found in all the samples, followed by Klebsiella spp. in 15 samples, Pseudomonas spp. in 14 samples, Bacillus spp. in 12 samples, Escherichia coli in 9 samples and Vibrio spp. in 7 samples. Salmonella spp. was detected in neither of the sample. 17 samples showed a high fungal load up to 107 cfu/g. Antibacterial activity of C. asiatica samples was demonstrated against eight laboratory isolates. Only four C. asiatica samples showed activity against Klebsiella spp. On the contrary, Aloe vera samples (12-14) showed antibacterial activity only against Staphylococcus spp.

Keywords: Centella asiatica; Aloe vera; Contamination; Antimicrobial activity

Location: Different areas of Dhaka city, Bangladesh

Start Date: 00-10-2015

End Date: 00-11-2015

Program Area: Pest Management

Commodity: Microbiological aspect, Medicinal Plants

Objectives:

Although there are several reports based on the determination of antibacterial activity of C. asiatica & A. vera, little is known about the harboring microorganism associated with these samples. Based on these facts and considerations, the recent study not only deals with the evaluation of antibacterial efficacy of the crude extracts of these herbal plants, but also to measure their level of bacterial contamination when collected from the market places from various areas of Dhaka city.

Methodology:

Collection of samples & processing. 10 Centella asiatica samples and 10 Aloe vera samples were collected randomly for the detection of microbiological analysis and antibacterial activity from different locations of Dhaka city within a period of October 2015 to November 2015. Samples were collected early in the morning and transported quickly to the laboratory according to the standard scheme. For preparing the sample suspension for microbiological examinations, 10g of each sample was weighed and homogenized in 90 ml normal saline to prepare 100ml sample suspension. Then, it was subjected to serial dilution (made up to 10-5 ) for microbiological analysis.

Total viable bacterial count (TVBC), total bacilli count and total fungal count. 0.1 ml from 10-2 & 10-5 dilution of each sample was spread over nutrient agar (NA) for TVBC & 0.1 ml from 10-2 dilution was spread over Starch agar and SDA plate for the detection of Bacillus spp. and total fungal load respectively.

Determination of Pseudomonas spp., Staphylococcus spp., Escherichia coli, & Klebsiella spp. 0.1 ml sample from 10-2 & 10-5 dilution was spread on Cetrimide agar, MSA (Mannitol Salt Agar), MAC (MacConkey Agar) for the determination of Pseudomonas spp., Staphylococcus spp. and coliform, respectively and incubated overnight at 37^oC.

Detection of Vibrio spp., Salmonella spp., and Shigella spp. As Vibrio spp., Salmonella spp. and Shigella spp. remain in the environment as viable but nonculturable (VBNC) state, they do not appear readily during microbiological test procedures. Before conducting serial dilutions, 1 ml of 10-2 diluted samples were enriched with alkaline peptone water (APW) for Vibrio spp. and selenite cystein broth (SCB) for Salmonella spp. & Shigella spp. at 37^oC for 2-4 hrs. The samples were then spread on the SS agar and TCBS agar for detecting Salmonella spp. (with black precipitates), Shigella spp. (reddish or pink color) & Vibrio spp. (small, yellow colored colony).

Determination of antibacterial activity of the herbal plants. The antibacterial activity of the samples was carried out by using agar well diffusion method. At first, the suspension (with standard turbidity compared to that of McFarland standard of 0.5) of each of the test bacteria (Escherichia coli, Bacillus spp., Staphylococcus spp., Vibrio spp., Salmonella spp., Pseudomonas spp., Klebsiella spp., & Listeria spp.) were spread evenly over the MHA using cotton swab which in turn resulted into uniform lawns. Wells made in the MHA was generated by cork-borer. Each of the samples then introduced separately in the specified well along with a positive control Gentamicin (GEN-10μg) and a negative control (normal saline). Existence of clear zone surrounding the sample solution (if any) was indicative of the presence of antibacterial activity of the samples tested.

Source of Information: Stamford Journal of Microbiology, 2016. Vol. 6, Issue 1, 39-43 ISSN: 2074-5346 (Print); 2408-8846 (Online)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In this study, a variety of bacterial load was observed in Centella asiatica and Aloe vera. Therefore, hygienic conditions must be enhanced in different stages of cultivation, produce, transfer, processing and a packaging. Considering the importance of medicinal plants in the human health and the large practice of the medicinal herbs in various forms for disease prevention and cure; culturing, harvesting, conveying and processing of these crops should be done in sanitary conditions. Thus, it is important to control environmental conditions and improvements in hygiene procedures during production and processing of herbs. A suitable sanitization method for disinfection before and when packaging medicinal plants would be recommended. In addition, a further investigation is necessary to assess antimicrobial activity by performing various solvent (ethanol extraction, methanol extraction, hot and cold-water extraction) extraction method.

Knowledge Management: Journal