Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effects of Sucrose, Ph and Temperature on in Vitro Rooting of Mulberry Microshoots of Morus Alba Cv S1

A Zaman
BSRTI, Rajshahi

R Islam
Rajshahi University

A C Barman
BSRTI, Rajshahi

A Ahad
BSRTI, Rajshahi

M A Qader
BARTI, Rajshahi

O I Joarder
Rajshahi University

Abstract/ Executive Summary:

The effects of sucrose, pH and culture room temperature on the development of roots from proliferated mulberry (cv S1) microshoots were evaluated. All the factors were found to have substantial effects on root induction of this mulberry cultivar. In the sucrose containing media, 30 g/l gave the best result. In the absence of sucrose roots formed were weak and slow growing. Root growth and multiplication rate were maximum when cultured at pH 5.5 and minimum being at pH 4.0. Adventitious root formation was improved by the shoots cultured at the ambient temperature of 30°C

Keywords: Mulberry,Effect, Temperature

Location: Bangladesh Sericulture Research and Training Institute

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Mulberry

Objectives:

Detail study on in vitro rooting of mulberry microshoots has not yet been studied. Present paper describes the effect of sucrose, pH and culture room temperature on in vitro rooting of mulberry microshoots.

Methodology:

Cultures of proliferating microshoots were established in full strength MS medium (Murashige and Skoog, 1962) using method communicated earlier (Zaman el. al. 1991, 1992a, 1992b). To get maximum number of shoots nodal explants from the initial in vitro proliferated microshoots were subcullured in cytokinin enriched MS medium in 250 ml conical flasks. After 5-6 weeks of culture shoots those reached upto 2-3 cm length were harvested and used for investigation. For optimising pH level microshoots having 2-3 cm in length were cultured in MS medium + 30 g/l sucrose + 6 g/l Difco-bacto agar + 1.0 mg/l indole butyric acid using six different (4.0. 4 *v 5 0. VS. 6.0 and 7.01 levels of pH. For determining the optimum sucrose concentration different concentrations (10,20,25,30,40 and 50 g/l) of sucrose were tested where pH of the medium was adjusted to 5.8 and agar was 6 g/l supplemented with IBA 1.0 mg/l. For choosing the suitable temperature, microshoots were cultured in -MS + 30 g/l sucrose + 6 g/1 agar + 1.0 mg/l IBA with pH 5.8. Culture tubes were incubated in four separate temperatures viz. 20, 25, 30 and 40°C All rooting microcuttings were cultured individually in 25x150 mm culture tubes with 15 ml of medium and were grown under 16 h photoperiod with light intensity of 2000 lux and humidity 55-60%. For optimizing pH level and sucrose concentration temperature was kept constant at 25.2°C

Source of Information: Bulletin of Sericulture Research 1993 vol 4:10-13 Published by Bangladesh Sericulture Research and Training Institute.

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Number of roots and root length were also promotive in higher temperature but at 40°C the growth of adventitious roots deteriorated comprehensively which indicates that in vitro mulberry microcuttings induced adventitious roots well in comparatively warm conditions rather than cold.

Knowledge Management: Journal