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Performance Testing and Molecular Characterization of BLRI Cattle Breed (bcb-1) Using Microsattelite Markers.

M. M. R. Mufti
Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka.

M. Q. Faruuqe
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202.

M. S. A. Bhyuian
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202.

K. S. Huque
Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka.

T. N. Nahar
Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka.

M. P. Mostari
Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka.

Abstract/ Executive Summary:

Bangladesh Livestock Institute Cattle Breed-1 (BCB-1) is a native cattle breed developed by Bangladesh Livestock Institute (BLRI), Savar, Dhaka. The BCB-1 is developed through selective breeding from indigenous cattle of active Brahmaputra and Jamuna flood plain agro-ecological region (AEZ-14) of Bangladesh. In this region, an assorted type of cattle named ‘Pabna’ was evolved through admixture of Hariana, Tharparker and Sahiwal genetic materials. BLRI collected these cattle and conserved Ex situ. Then selective breeding programme was undertaken with the goal of improvement of milk and meat production performance of the germ-plasm. Hence, the animals of the breeding herd were selected based on lactation yield and live weight over the last 20 years. Meanwhile, the physical appearance and production performance of the cattle is changed to a district feature than that of their progenitor. The phenotype and performances characteristics of BCB-1 have done, but the molecular characterization of this cattle yet to be done. Therefore, the project was undertaken with the objectives of identify the genetic diversity within BCB-1 cattle and to test the performance of BCB-1 at farm community. To achieve the first objective, 40 blood samples were collected form BCB-1 and subsequently, DNA was extracted from these samples using DNA extraction kit. The banding patterns indicate the extracted DNA from each sample is free from any contaminant and suitable for any molecular technique. The extracted DNA will be used for molecular characterization of BCB-1 in next year. For performances testing of BCB-1, 17 cows and heifer and 2 bulls were distributed among 17 farmers of Panchbibi upazila under Joypurhat and Badargonj upazila of Rangpur district. The farmers are responsible to collect records and the performance was evaluated on the basis of their records. Cows and heifers delivered calves producing more milk than their existing native stock under similar management practices. The study revealed that the farmers of the selected communities were more interested to rear the BCB-1 germplasm.

Keywords: BCB-1, Performance test, Molecular characterization

Location:

Start Date: 01-07- 2009

End Date: 30-06-2010

Program Area: Variety and Species

Commodity: Cattle

Objectives:

1. Evaluation of performance of BLRI Cattle Breed-1 (BCB-1) at farm levels/farmers management system.

2. Molecular characterization of BCB-1 using microsattelite markers.

Methodology:

Activity 1: Performance testing BCB-1 at farm levels The institute through Public-Private Partnership with IC-SAAKTI distributed 12 heifers, 5 cows of different ages and 2 breeding bulls to the farmer’s community given in Table 1. The communities were located in Cit Laxhampur, Badargonj upazila, Rangpur and Nilta, Panchbibi upazila, Joypurhat,. IC-SAAKTI worked in these communities with the help of its local partner organizations; CTW (Come to work) for Badargonj and ASPES ( Agroforestry Service Provider Extension Sangstha) for Panchbibi. BLRI distributed their BCB-1 cattle to the farmers on payment basis. BLRI started its dissemination programme with IC-SAAKTI under the following terms and conditions: (1) Cow/heifers should be inseminated / bred by BLRI supplied bulls only. No other bulls should be used for breeding of the supplied cow/heifers. (2) Supplied animals should not be sold out, slaughtered or exchanged with other farmers. (3) Farmers will maintain production, reproduction and health records under direct supervision of SAAKTI technical personnel. The goal of IC-SAAKTI was to supply an improved sustainable deshi cattle breed, increase milk and meat production and finally to increase income for the community farmers. Each of the 19 farmers in two communities received a cow or a heifer, a farmer from a community received a BCB-1 bull for serving the BCB-1 and other native heifers or cows on payment basis. Activity 2: Molecular characterization of BCB-1 using microsattelite markers. Eighty blood and milk samples were collected from RCC and BCB-1 from BLRI research farm and RCC habitat in Chittagong. The whole methodology involved several steps are as follows: 1. Collection of blood: Blood samples were collected by 10 ml syringe and kept in EDTA containing collection vial. The vials were gently tilting for mixing the blood with anticoagulant. The vial was marked with the date of collection, sex and genotype. During blood collection the vial was put into an ice box for preservation and processing was completed within 5 hours. The vial was then stored at -20? temperature for long time preservation. 2. Extraction of DNA: DNA was extracted using Easy-DNATM kit ( Invitrogen, USA) and the whole extraction protocol given below: (1) Pipette 350 µl of blood into each micro-centrifuge tube. Blood samples mix to from a homogenous solution. (2) Add 500 µl of solution A to the tube. Mix by inversion several times. (3) Incubate at 65? for 6 minutes. (4) Remove sample from heat block or water bath and mix by inversion. (5) Add 900 µl of chloroform and vertex vigorously. The mixing is complete when the liquid portion of the sample flows freely and the hemoglobin looks like small chocolate-covered particles. (6) Add 500 µl of solution and vertex briefly until the sample is uniformly viscous. (7) Centrifuge at 14,000 rpm in a micro-centrifuge for 10 minutes at room temperature. (8) Pipette the clear, aqueous phase into a new 1.5 ml micro-centrifuge tube. (9) Add 1 ml of room temperature 100% ethanol and mix by inversion until a precipitate forms. A precipitate is usually seen within 30-60 seconds. (10) Centrifuge at 14,000 rpm in a micro-centrifuge for 5 minutes at room temperature. (11) Decant the supernatant and add 1 ml of room temperature 70% ethanol. (12) Centrifuge at 14,000 rpm in a micro-centrifuge for 2 minutes at room temperature. (13) Decant the supernatant. Centrifuge at 14,000 rpm in a micro-centrifuge for 1 minute at room temperature. (14) Remove residual ethanol with a pipette and invert tubes to dry. (15) Add 100-150 µl of autoclaved nuclease-free water to each tube. (16) Incubate at 65? for 5 minutes.

Source of Information: BLRI. Proceedings of the Annual Research Review Workshop 2009-2010, Bangladesh Livestock Research Institute, Savar, Dhaka 1341.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Cows and heifers delivered calves producing more milk than their existing native stock under similar management practices. The study revealed that the farmers of the selected communities were more interested to rear the BCB-1 germplasm. This programme requires to expand in other areas with other interested organizations for getting expected outputs in plain and char land and also in coastal areas. The evidence of extracted DNA indicates that it was free from any contamination and could be used in any molecular study like micro-sattelite marker system. The research is on-going and the continuous monitoring and execution is needed to get the actual performance information of BCB-1 at farmer’s condition. The molecular characterization of BCB-1 was not completed due to some problems and would be done in the following year.

Knowledge Management: Report/Proceedings