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Effect of Ga3 on Male Sterility of Onion

M N A Chowdhury
Senior Scientific Officer
Spices Research Center,BARI, Shibganj, Bogra

, R Ara
Senior Scientific Officer
Spices Research Center,BARI, Shibganj, Bogra

M. S. Alam
Senior Scientific Officer
Spices Research Center,BARI, Shibganj, Bogra

M Hasan
Scientific Officer
Spices Research Center,BARI, Shibganj, Bogra

B Sarkar
Scientific Officer
Spices Research Center,BARI, Shibganj, Bogra

M Ratna
Scientific Officer
Spices Research Sub-Center,BARI, Faridpur

Abstract/ Executive Summary:

The experiment was conducted at Spices Research Cente, Bogra during robi season (2012-2013) to find out the male sterile line for hybrid variety development of onion with desired traits by using GA₃. The planting materials were BARI Onion-1, 2, 3, 4 &5. The treatments were viz. T1 = GA3 (300 ppm) at 30 days after sowing (DAS) T2 = GA3 (300 ppm) at 30+ 45 DAS T3 = GA3 (300 ppm) at 30 + 45 + 60 DAS T4 = GA3 (400 ppm) at 30 DAS T5 = GA₃ (400 ppm) at 30 + 45 DAS T6 = GA₃ (400 ppm) at 30 + 45 + 60 DAS T7 = GA₃ (500 ppm) at 30 DAS T8 = GA₃(500 ppm) at 30 + 45 DAS T9 = GA₃ (500 ppm) at 30 + 45 + 60 DAS and T10 = Water spray. Spraying of GA₃according to treatments was done. It was observed that some treatment with different variety showed 20-40% male sterility. It was not up to the mark. It was suggested that the trail may be repeated in the next year with treatment modification.

Keywords: GA3, MALE STERILITY, INDUCTION, ONION.

Location: Spices Research Center,BARI, Shibganj, Bogra

Start Date: 01-11-2012

End Date: 30-04-2013

Program Area: Variety and Species

Commodity: Onion

Objectives:

To develop the male sterile line for hybrid variety development of onion with desired traits by using GA₃.

Methodology:

The experiment was conducted at Spices Research Cente, Bogra during rabi season (2012 -2013) to find out the male sterile line for hybrid variety development of onion with desired traits by using GA₃. The planting materials were BARI Onion-1, 2, 3, 4 & 5. The experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. The treatments were viz. T1 = GA₃ (300 ppm) at 30 days after sowing (DAS),T2 = GA₃ (300 ppm) at 30+ 45 DAS, T3 = GA₃ (300 ppm) at 30 + 45 + 60 DAS, T4 = GA₃ (400 ppm) at 30 DAS, T5 = GA₃ (400 ppm) at 30 + 45 DAS, T6 = GA₃ (400 ppm) at 30 + 45 + 60 DAS, T7 = GA3 (500 ppm) at 30 DAS, T8 = GA₃(500 ppm) at 30 + 45 DAS, T9 = GA3 (500 ppm) at 30 + 45 + 60 DAS and T10 = Water spray. The crop was planted on 13 November 2012 and harvested on 05 May 2013. The size of unit plot was 2.0 m x 2.0 m having plant spacing of 25 cm x 20 cm. Onion was fertilized with recommended dose of cow dung 10 t/ha, N115, P54, K75, S20, Zn3 and B2 Kg/ha.Pollen collection and pollen sterility identification In all treatments, fresh pollen was collected from the field of effective opened anther of treated plants. A tiny droplet of 1% acetocarmine stain was placed on the slide. Using a teasing needle, a small amount of pollen was be placed on stain and thoroughly mixed to ensure uniform penetration of the stain into the pollen.

Source of Information: Annual Research Report 2012-13, Spice Research Center, BARI, Shibganj, Bogra

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 80,000/-

Total Budget (Tk.) : 80,000/-

Achievement/Findings:

Spraying of GA3 according to treatments was done. It was found that treatment T9 showed about 30% male sterility in case of BARI Onion-1 & 4. Only 20-25% male sterility on BARI Onion-2 & 5 was recorded in treatment T8. About 50% male sterility was observed from T9 treatment in BARI Onion-3. It was not up to the desired mark.

Knowledge Management: Report/Proceedings