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Isolation and Identification of Circulating Salmonella Serovars from Chicken in Bangladesh

A. A. Bhuyan
Scientific Officer
Poultry Technology Development and Dissemination Project, BLRI, Savar, Dhaka-1314.

M. Giasuddin
Senior Scientific Officer
Animal Health Research Division, BLRI, Savar, Dhaka-1314.

M. N. Islam
Principal Scientific Officer
Poultry Production Research Division, Animal Health Research Division, BLRI, Savar, Dhaka-1314.

A. A. Bhuiyan
Scientific Officer
Poultry Production Research Division, Animal Health Research Division, Savar, Dhaka-1314.

Abstract/ Executive Summary:

The study was conducted to isolate and identify Salmonella serovars circulating at poultry production areas. For these, a total of 260 cloacal swabs, 124 feed, 124 water, 124 litter samples as well as 18 dead birds were collected from 8 Technology Dissemination Area (TDA) located at eight different district of Bangladesh. Collected samples from poultry and poultry farms were first inoculated into tetrathionate broth base (TBB) and incubated at 37°C for 24 hours followed by inoculation of McConkey agar plates to separate Salmonella spp from other bacteria. The isolates were identified by observing their colony characters on BGA, SSA, and DHL agar plates. Staining characteristics were examined using Gram’s staining method. Motility of the organisms were detected by the “Hanging Drop Slide” examination. Isolated organism with supporting growth characteristic of Salmonella on various media were subjected to biochemical tests such as Sugar fermentation test and Triple sugar iron agar slant reaction. A total of 128 samples were found positive for Salmonella spp and by the motility test 21 out of 128 isolates were found to be motility. Serotyping was done according to the Kauffmann White Scheme. Among the 128 isolates. 117 isolates was found posit8ive for Group D1, 5 for Group B while 6 isolates was positive for Group (C2-C3).

Keywords: Salmonella, Poultry farms, ChickenWhite Scheme.

Location: 8 different district of Bangladesh

Start Date: 01-07-2010

End Date: 30-06-2011

Program Area: Animal Health and Management

Commodity: Chicken, Chicken

Objectives:

To isolate and identify Salmonella serovars circulating at poultry production areas. Isolated organism with supporting growth characteristic of Salmonella on various media were subjected to biochemical tests such as Sugar fermentation test and Triple sugar iron agar slant reaction.

Methodology:

Cloacal swab from live bird, feed, water and litter from poultry farms from TDA areas were collected and were examined for routine bacteriology using the procedure. The isolation was done using TBBB incubated at 37°C for 24 hours followed by inoculation into MacConkey agar to obtain pure colonies of Salmonella spp. The isolates were identified by observing their colony characters in BGA, SSA, and DHL agar. Staining characteristics were examined using Gram’s staining method. Motility of the organism was detected by the “Hanging Drop Slide” examination. Isolated organisms with supporting growth characteristic of Salmonella on various media was subjected to the following biochemical tests such as sugar fermentation test and triple sugar iron agar slant reaction. After isolation and identification of Salmonella spp, pure colony was collected by inoculating into SSA for several times. Laboratory methods for the identification of Salmonella serovars include serotyping, which is performing by the identification of O, H1, and H2 antigens according to the Kauffmann-White Scheme.

Source of Information: BLRI. Proceedings of Annual Research Review Workshop 2010-2011. Bangladesh Livestock Research Institute, Savar, Dhaka-1341

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Poultry products and derivatives have been concerned with zoonotic Salmonella in both developed and developing countries. Pullorum disease with Salmonella Pullorum represented a serious poultry health problem until huge resources were used to limit its expansion, but these efforts allowed the emergence of other Salmonella spp. The development of food-borne disease in humans caused by S. Typhimurium, and S. Enteritidis following the consumption of contaminated eggs and egg products is mainly due to lack of hygiene practices.

Knowledge Management: Report/Proceedings