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Viability Testing, Testing, Periodic Evaluation and Routine Monitoring of Stored Germplasm.

Mir Sharfuddin Ahmed
SO
Genetic Resources And Seed Division,BRRI, Gazipur

Abstract/ Executive Summary:

Objective of the study was to check and monitor the germination (%) of the stored germplasm in the short term and medium term storage. The viability of the stored germplasm was monitored from randomly selected germplasm in three seasons. 150 accessions in Boro season, 200 accessions in T. Aman and 150 accessions in Aus season were checked randomly for viability test during 2004-05

Keywords: Short term, Boro season, Germination (%), Stored germplasm, Viability

Location: GRS Division, BRRI

Start Date: 01-07-2004

End Date: 30-06-2005

Program Area: Variety and Species

Commodity: Rice

Objectives:

To check and monitor the germination (%) of the stored germplasm in the short term and medium term storages.

Methodology:

Seed viability was monitored by testing germination percentage of the stored germplasm in short term and testers in the medium storages. Five varieties viz: Dharial, Hashikalmi, Nizersail, Patnai 23 and Purbachi possessing a seed viability range from very high to very low were used as testers in the medium term storage and viability was measured on every six month interval usually on July and December of every year for prediction of the viability of mid-term storage germplasm. Silica gel was used to maintain the moisture/relative humidity in the seed container. The color of silica gel was changed from blue to pinkish, and then the silica gel were taken out and replaced by the fresh blue silica gel.

Source of Information: BRRI. Proceedings of the BRRI Annual Internal Review for 2004-2005. Bangladesh Rice Research Institute (BRRI). Gazipur -1701.

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The viability of the stored germplasm was monitored from randomly selected germplasm in three seasons. 150 accessions in Boro season, 200 accessions in T. Aman and 150 accessions in Aus season were checked randomly for viability test during 2004-05 in short term storage and most of the tested germplasm possessed about 80% germination. The germplasm accessions rejuvenated and stored during 2004-05 in short term were found to be more than 80% germination except 3 and 4 accessions in Aman and Boro seasons, respectively. The germination percentages of the 5 test samples in the medium term storage are presented in table 4c. The germination percent of tested varieties varied, ranging from 22-89% in December 2004. It can be concluded that the rice germplasm which were stored before eighty’s still possess good viability in the medium term conditions.

Knowledge Management: Report/Proceedings