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Study on Efficacy, Potency and Safety of BLRI Developed Enterotoxaemia Toxoid

M. Ershaduzzaman
Goat and Sheep Production Research Division, BLRI, Savar, Dhaka-1341

M.J.F.A. Taimur
Animal Health Research Division, BLRI, Savar, Dhaka-1341

Md. Shahin Alam
Goat and Sheep Production Research Division, BLRI, Savar, Dhaka-1341

A.T.M. Mahbub-E-Elahi
Department of Microbiology, Sylhet Agricultural University, Tilagor, Sylhet

Abstract/ Executive Summary:

The study was undertaken to evaluate the efficacy, potency and safety of the BLRI developed toxoid. Fresh toxin was prepared from the local toxigenic strains of Cl. Perfringens type D. The strength (i.e. concentration) of the toxin was calculated by “Absorbance Assay” on a spectrophotometer. The toxicity of the prepared toxin was tested in adult mice and the mice lethal dose (LD) was calculated. Subsequently, toxoid was prepared against type D enterotoxaemia and its efficacy was tested in mice. The calculated concentration of the prepared toxin was 14.4 mg/ml. The oral LD50 and LD100 for mice of the prepared toxin were as 21.6 µg and 36 µg, respectively. BLRI developed toxoid offered significant protection and found effective in 92.5% mice experimental challenges. Therefore, BLRI developed enterotoxaemia toxoid could be an effective tool to prevent and control enterotoxaemia in sheep and goat, though it needs to be validated in natural hosts.

Keywords: Clostridium perfringens toxins, Goat enterotoxaemia, Toxoid, Sheep and Goat

Location: Laboratory of the Goat and sheep production research division, BLRI

Start Date: 01-07-2011

End Date: 30-06-2012

Program Area: Pest Management

Commodity: Sheep

Objectives:

1. The study was undertaken to evaluate the efficacy, potency and safety of the BLRI developed toxoid. 2. Efficiency of BLRI developed enterotoxaemia to prevent and control enterotoxaemia in sheep and goat. .

Methodology:

Preparation and Separation of toxin Fresh toxin was prepared from the local toxigenic strains of Cl. Perfringens type D. Development of toxoid The toxoid or vaccine was prepared from the isolated strain of Cl. Perfringens type D enzootic in Bangladesh. Determination of the strength and lethal dose (LD) of the toxin and efficacy of the toxoid The strength (i.e. concentration) of the toxin was calculated by “Absorbance Assay” on a spectrophotometer using the absorbance at 280 mm following the formula. Determination of the efficiency of the toxoid The BLRI toxoid was certified as sterile after ensuring the absence of microbial contamination and tested in mice to ensure its safety.

Source of Information: BLRI. Proceedings of Annual Research Review Workshop, 2011-2012. Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka-1341.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Toxins were separated from the major causal type of the organism and the strength as well as the mice lethal dose (LD) of the toxin was determined. Subsequently, toxoid was prepared against type D enterotoxaemia and its efficiency was tested in mice. BLRI developed toxoid offered significant protection and found to be effective in 92.5% mice in experimental challenge infection. The findings were presented at BLRI annual workshop 2011-12. According to the comments and suggestions, the study has been further designed to meet the real need in the field.

Knowledge Management: Report/Proceedings