Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Development and Feeding Effect of Ca-salt of Fatty Acids in Dairy Cows (activity 7).

N. R. Sarker
Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka 1341.

H. Islam
Department of Animal Nutrition, Bangladesh Agricultural University, Mymensingh-2202

M. M. Rahman
Department of Dairy Science,Bangladesh Agricultural University, Mymensingh-2202

K. S. Huque
Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka 1341.

Abstract/ Executive Summary:

In our country, farmers usually fed paddy straw based ration to their animals which is deficient in Ca, as a results all the animals are suffering from Ca deficiency. To overcome this problem, an attempt was made to develop Ca salts of fatty acids for the benefit of animals. Feeding diets supplemented with Ca salts of fatty acids is a practical way to bypass rumen biohydrogenation. Studies have already shown that diets supplemented with Ca salts of fatty acids of CLA (Ca-CLA) effectively reduce milk fat percentage and yield in lactating cows (Giesy et al., 2002; Perfield II et al., 2002; Bernal Santos et al., 2003). Dietary Ca salts tFA (Ca-tFA) supplementation could potentially increase the postruminal availability of trans-18:1 fatty acids and provide an alternative to abomasal infusion to test the potential effects of trans-18:1 fatty acids on milk fat synthesis. A current interest in milk fat manipulation is to produce milk with higher proportions of unsaturated fatty acids. Milk with higher proportions of unsaturated fatty is likely to be performed by the consumer because of concerns about too much saturated fat in the diet. An increase in saturated fatty acid intake has been linked to a rise in cardiovascular disease. Due to increasing demand for value-added foods with health benefits, strategies have been initiated to produce dairy products enriched with rumenic cis(c)-9, trans(t)-11-18:2 and vaccine ruminants, has been reported to protect and moderate against certain cancers, diabetes, immunity, atherosclerosis, bone growth and obesity (Ip et al., 2003). Vaccine acid is produced in rumen bacteria by the action of isomerases and reductases from dietry polyunsaturated fatty acids (PUFA) and is subsequently converted to c9, t11-18:2 by 9-desaturase in the tissues of ruminants (Griinari et al., 2000). With above view and to determine the effect of feeding Ca- salts of fatty on milk production and quality, two studies were conducted.

Keywords: Development, Feeding effect, Ca-salt, Fatty acids, Dairy cows.

Location: Animal Production Research Division, Bangladesh Livestock Research Institute, Savar

Start Date: 01-07-2009

End Date: 30-06-2010

Program Area: Animal Health and Management

Commodity: Straw

Objectives:

1. Development of Calcium salts of fatty acids with vegetables oil. 2. Study the feeding effect of Calcium salts of fatty acids on dry matter intake, milk yield and composition, digestibility and microbial protein synthesis. 3. Study the effect of Calcium salts of fatty acid on acid profile of milk fat. 4. Recommended suitable level of supplementation of Calcium salts of fatty acid for optimum productivity of dairy animal.

Methodology:

1. Site of the experiment: The experiment was conducted at the Animal Production Research Division, Bangladesh Livestock Research Institute, Savar during for a period of five month from 1st July to 1st December 2009. The chemical analysis of feed was done in the Animal Nutrition Laboratory of BLRI, Soil Science Department for Calcium (Ca) analysis, Central Laboratory of Bangladesh Agricultural University (BAU) Mymensingh for sodium (Na) analysis and also the fatty acid profile was performed in Institute of Food Science and Technology (IFST). Protozoa count of rumen liquor was performed at Rumen Microbiology Laboratory of Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka. Feeding trial was done at Sheep and Goat farm of Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka. 2. The research work consists of two experiment: (1) Preparation of Calcium salts of fatty acid and (2) Feeding trial after obtaining the best results from the first experiment. Expt. 1: Preparation of Calcium salts of fatty acid Four parts of an aqueous solution of NaOH (6M) were then added to five parts of oil, and the hydrolysis of oil triacylglycerols was performed at 95 to 100? with continuous agitation and bubbling N. When no more oil was visible, the resulting blend was left to stand at 5? until Na soaps to water, and a saturated. The Na soaps then were dissolved in hot water (95 to 100?) using a 1:5.6 ratio of soap to water and a saturated solution of CaCI2 at a ratio of 2.5 parts and 4.5 parts of soap to water was added for salting out. A filter cloth was used to filter the Ca salts, and tap water was used to remove residual NaOH and excess CaCI2. The Ca salts were finally dried both in air and sun, and kept at about ñ20? until use for feeding. Expt. 2: Feeding trial Animal selection: Ten female Sheep with an average live weight of 12.955±2.766 Kg were selected for the experiment. The experiment was last for last 15 days. The animals were divided into two groups of five sheep in each group named control group T0 live weight 12.92±3.02 and treatment group T1 12.99±2.844. The animals were housed in individual plan. Dietary treatments: During the trial period sheep belonged to treatment group T0 were offered Napier silage mixed with molasses (for increasing appetite) adlibitum and concentrate mixture 1.5% of the live weight of the individual animal. Sheep belonged to treatment group T1 were offered the same diet but this group also offered 3% Calcium salts of fatty acid on the basis of concentrate mixture. During the whole period fresh and clean drinking water was available to all the animals. Diets were offered two times daily. In the morning (8:00 am) animals were offered half of the concentrate mixture and in evening (2:00pm) the next half. In the last five days of experimental period 3% Calcium salts of fatty acid on the basis of concentrate mixture were given with the morning portion of concentrate mixture. Napier silage mixed with molasses were given only at 10:00 am daily. Each morning left over feed samples were weighed to measure the feed intake. No refusals of concentrate mixture were found in any case. Design and layout of the experiment: The experiment was conducted in a Completely Randomized Design (CRD). Sample collection: Rumen liquor were collected by using saline pipe inserted in another wider pipe and drawn by syringe. After collecting PH were taken and then it was taken in 10 ml tets tube at a ratio of 1:9 rumen liquor and MFS stain respectively for protocol count and finally it were analyzed for rumen Ammonia Nitrozen Concentration. Preparation of MFS (Methyl green-formalin-sodium chloride) stain/ Preservatives Ingredient: (1) 35% 100ml Formaldehyde (2) 900 ml distilled water (3) 0.6g Methyl green (4) 8.0g sodium chloride (Lab Grade). Statistical analysis: The data were evaluated statistically using MS stat software.

Source of Information: BLRI. Proceedings of the Annual Research Review Workshop 2009-2010, Bangladesh Livestock Research Institute, Savar, Dhaka 1341.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Expt 1: Calcium salts of fatty acid were prepared by adding 3 different level of CaCI2 and then different drying process (air and sun) were measured in the present study and found that 3.5 parts CaC12 give the best results in either of the drying processes. Thus Calcium salts of fatty acid can be prepared by applying 3.5 parts CaC12 and it can be dried in either drying process.

Expt 2: Calcium salts of fatty acid can significantly reduce protozoal number without affecting the rumen PH and ammonia nitrogen. In this study Calcium salts of fatty acid were used only one level (3% of the concentrate mixture). Different level of Calcium salts of fatty acid could be used to obtain the best combination results.

Knowledge Management: Report/Proceedings