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Effect of Temperature and Ph on Growth and Sporulation of Fusarium Moniliforme

Md. Shahadat Hossain
Senior Scientific Officer
Training DivisionBangladesh Rice Research Institute, Gazipur 1701

Dr. M Ayub Ali
Professor
Department of Plant Pathology, Bangladesh Agriculture University Mymensingh

Dr. MA Taher Mia
Ex. CSO
Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701

Abstract/ Executive Summary:

Culture of the fungus at 25^oC produced the widest colony at all the period of measurement and are significantly higher than the other level of temperature. The optimum temperature for growth was 25^oC where colony diameter of 30.58, 58.96 and 75.87 mm were widest for all period of measurement. The fungus did not grow at 5^oC regardless of the length of incubation. Colony diameter at 30^oC on the sixth day was significantly lower than those of 25^oC. However, both temperature levels at third and ninth days were statistically identical at all levels of temperature except 5^o and 35^oC.At all the temperature levels, culture at 30^oC consistently produced the highest total number of spore (50.05 x 106) after nine days of incubation. It was 24.69 x 106 at 25^oC. The sporulation was 3.98 x 106 and 8.58 x 106 at 15 and 20^oC respectively. Only 2.75 x 106 spores per ml was produced at 35^oC. Since the fungus did not grow at 5^oC sporulation likewise did not occur

Keywords: Temperature, pH, Growth, Sporulation, Fusarium moniliforme

Location: BRRI HQ

Start Date: 01-07-2011

End Date: 30-06-2012

Program Area: Pest Management

Commodity: Rice

Objectives:

To determine the optimum temperature and pH level for growth and sporulation of F. moniliforme.

Methodology:

Effect of temperature on growth: By means of core borer, disc from an 8-day old culture of the fungus grown in 10 ml medium were cut proximal to the edge of the colony. Each disc was transferred to the center of a 20 ml plated medium. Plates were wrapped with two sheets of carbon paper to exclude light effect and incubated at 5^o, 15^o, 20^o, 25^o, 30^o and 35^oC temperature in incubator. Colony diameter in mm were measured on the third, sixth and ninth day of incubation and sporulation was measured on ninth day. For each period of measurement and temperature level, four replications were made. Colony diameter was measured at the widest point and the average taken minus 5mm diameter of the culture disc used. Determine of sporulation: To determine sporulation, 10 ml sterile distilled water was added to each culture and the surface growth was scraped gently without scarifying the agar. The suspension was placed in a test tube and shaken 25 times in an up an down movement over a distance of one foot. Mycelial growth was removed and dilutions were made to facilitate counting of spore. Number of spores per ml was taken by means of a hemacytometer. Effect of pH on growth: Adequate PDA medium were prepared and poured into nine separate conical flask. The pH of these nine conical flask were adjusted to seven different pH level such as 4, 5, 6, 7, 8, 9 and 10 with 1.0% lactic acid and 10% sodium hydro oxide solution. It should be mentioned that the accuracy range of the pH meter was ± 0.1. All conical flask with media were sterilized at 121^oC for 15 minutes in an autoclave. For each pH level 20ml PDA media per sterilized petri plates was poured Four mm diameter of young culture blocks of F. moniliforme were placed centrally on the patri plates. All the plates incubated at room temperature. Colony diameter in mm were measured on the third, sixth and ninth day of incubation and sporulation was determine after nine days. For each period of measurement and temperature level, four replications were made. Determine of sporulation: Determine of sporulation was as same as described above.

Source of Information: Proceedings of the BRRI Annual Internal Research Review, 2011-2012

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The optimum temperature for growth was 25^oC where colony diameter of 30.58, 58.96 and 75.87 mm were widest for all period of measurement. The fungus did not grow at 5^oC regardless of the length of incubation. Colony diameter at 30^oC on the sixth day was significantly lower than those of 25^oC. However, both temperature levels at third and ninth days were statistically identical at all levels of temperature except 5^o and 35^oC. The sporulation was 3.98 x 106 and 8.58 x 106 at 15 and 20^oC respectively. Only 2.75 x 106 spores per ml was produced at 35^oC. Since the fungus did not grow at 5^oC sporulation likewise did not occur.

Knowledge Management: Report/Proceedings