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Standardization of High Frequency Regeneration of Existing Genotypes of Jute (corchorus Olitorius) in Bangladesh

Mst. Naznin Pervin
Scientific Officer
Capsularis DepartmentBreeding DivisionBangladesh Jute Research InstituteManikmia Avenue, Dhaka-1207

Dr. Lutful Hassan
Professor
Department of Genetics and Plant BreedingBangladesh Agricultural University, Mymensingh-2202, Bangladesh

M.A. Haque
Department of Genetics and Plant BreedingBangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Amirul Alam
Department of Genetics and Plant BreedingBangladesh Agricultural University, Mymensingh-2202, Bangladesh

A.H.M. Kamal
Department of Genetics and Plant BreedingBangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

Four varieties of Corchorus olitorius were used to observe their regeneration potentiallity and to establish a suitable in vitro plantlet regeneration protocol of jute. Cotyledons were used as explants. MS medium supplemented with different phytohormone concentration and combinations were used to observe the callus induction ability of the explants. The highest callus induction (88.56%) was observed in O-9897 with the media supplemented with 3 mg/L BAP + 0.5 mg/L IAA. Calli were maintained to get sufficient number of regenerants. The highest percentage of regenerants were found O-9897 (88.94%) followed by O-72 (85.83%) and OM-1 (72.61%). Among the phytohormone combinations, MS medium supplemented with 3 mg/L BAP + 0.5 mg/L IAA showed the highest shoot regeneration (97.78%). The root formation from regenerants was the best (69.91%) on half strength of MS media supplemented with 0.6 mg/L IBA. The in vitro regenarated plantlets from the genotypes O-9897, O-72, OM-1 and O-4 could be established in the field successfully.

Keywords: Standardization, In vitro regeneration, Genotype, Jute, explants, Phytohormones

Location: Plant breeding and Genetics Laboratory of Bangladesh Agricultural University, Mymensingh.

Start Date: 01-01-2007

End Date: 31-12-2007

Program Area: Variety and Species

Commodity: Jute

Objectives:

1. Optimization of in vitro regeneration performance of jute varieties.

2. Standardization of a protocol for callus induction and shoot regeneration with different hormonal treatments. and

3. Screening of some genotype of Corchorus olitorius L. for their tissue culture potentiality.

Methodology:

Experimental materials : Healthy seeds of four varieties of Corchorus olitorius L. (O-9897, O-72, O-4 and OM-1) were used as experimental materials. All the experimental materials were sterilized before use. Media: The seedlings raised in axenic culture were used as the source of different kinds of explants. The hypocotyls and cotyledonary nodal segments (cotyledons) were used as explants. The explants were first cultultured on agar solidified MS medium supplemented with 1.0, 2.0, 3.0, 4.0 and 5.0 mg/L BAP with constant concentration of 0.5 mg/L IAA for callus induction and shoot regeneration. When those shoots were about 2-3 cm in length, they were rescued from the vials aseptically inside the laminar air flow cabinet, and were again cultured in vials with freshy prepared root induction medium containig half strength MS medium supplemented with 0.2, 0.4 and 0.6 mg/L IBA for root formation.Culture technique: Sterilized seeds were placed into sterilized seed germination medium in culture vessels. Twenty five seeds were then placed in each vials. The culture was then incubated in dark till the germination of seeds. These were transferred to 16 hours light for normal seedlings growth. Seven days old seedlings were used as source of contamination-free explants. The seedlings raised in axenic culture were used as source of different kinds of explants. The hypocotyls and cotyledonary nodal segments (cotyledons) were used as explants. Attempts have been taken for the induction of embryogenesis and organogenesis using different explants in MS medium supplemented with different hormones. After the calli attained a size of about 20-25 mm in diameter, they removed aseptically and sub-cultured to other vials, the calli were again cultured on freshy prepared medium containing the same hormonal supplements, for shoot induction from the cells. When shoots were about 2-3 cm in length, they were rescued from the vials aseptically inside the laminar air flow cabinet, and were again cultured in vials with freshy prepared root induction medium to form root.

Source of Information: Journal of Agroforestry and Environment: 2(1): 83-86, 2008.

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It may be concluded that among four varieties of Jute (Corchorus olitorius L.) O-9897 performed best and this findingg will be helpful in different biotechnological approaches.

Knowledge Management: Journal