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Isolation of Desirable Genetic Variants of Corchorus Capsularis Through Cotyledon Culture and Their Utilization in Breeding Programme for Varietal Improvement.

Dr. Asma khatun
chief Scientific officer
1012-2560 Kingston Road, Scarborough, Ontario, M1M 1L8, Canada

Zabun naher

Abstract/ Executive Summary:

In vitro grown cotyledons of 10 varieties of C. capsularis (eg. CVL-1,D-154, CVE-3,CC-45,BJC-83,BJC-718,BJC-2142,BJC-7370, Tricap-1 and Tricap-2. A large number of shoots produced at the cut end of the petiole of each cotyledon of all varieties. The rate of plant regeneration from the cotyledons and rate of shoot multiplication per cotyledon are shown differently. The maximum number of cotyledon responded for shoot production was from CVE-3 and Tricap-2 which make 90 and 91 percent respectively. However highest numbers of shoots produced per cotyledon was recorded from Tricap-2 which are 32 shoots (average) per cotyledon. The regenerated plants were rooted in vitro and then transferred in pots in glass house premises. The plants grow well into maturity and produced fruits. No major somaclonal variation was noticed

Keywords: Cotyledon, Regeneration, somaclonal varation

Location: Tissue culture and Genetic Engineering Laboratory, Cytogenetics department, BJRI

Start Date: 01-01-2001

End Date: 31-12-2003

Program Area: Variety and Species

Commodity: Jute

Objectives:

a) To establish an efficient plant regeneration system from the petoler tissue of cotyledon and shoot apices of C. capsularis. b)To select the proper tissues for subsequent culture and will have application in plant genetic engineering technology.

Methodology:

1. Cotyledon (with attached petioles) of in vitro grown seedlings (7 day old ) and shoot apices of the cultured in vitro grown shoots of C. Capsularis would be cultured on agar solidified MS medium supplemented by auxinsand cytokynins 2. Cultures should be maintained in a growth room with 28° c and a photoperiod of 12 hour 3. Transfer regenerated shoots in pots. 4. Collection of seeds from the regenerated plants 5. Each experiment should be repeated 3 times using minimum of 3 replicates.

Source of Information: Annual research report 2001-2002, BJRI

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 5000 per year

Total Budget (Tk.) : 5000 per year

Achievement/Findings:

An efficient plant regeneration system were established from the petiolar tissue of cotyledons and shoot apices of C. capsularis at BJRI tissue culture laboratory.

Knowledge Management: Report/Proceedings