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Production and Application of Ligninase for the Improvement of the Brightness of Jute Fibre / Fabrics

Mrs. Suraiya Akhter SSO
SSO
Biochemistry Department, BJRI ,Dhaka - 1207

Md. Mozibur Rahman
SO
Biochemistry Department, BJRI ,Dhaka - 1207

Dr. S.H. Talukder
CSO
Biochemistry Department, BJRI ,Dhaka - 1207

Abstract/ Executive Summary:

Two strains of white – rot fungi P. chrysosporium and S. pulverulentum were choosen for this experiment. About 7 strains have been isolated. Ligninase activity of two fungal strains have been ascertained.

Keywords: Ligninase , Fibre brightness, Fermentation , Enzymes

Location: Central station, BJRI , Dhaka

Start Date: 01-01-2000

End Date: 01-07-2001

Program Area: Knowledge Management

Commodity: Jute

Objectives:

Product development for diversified uses of jute product both horizontal and vertical .

Methodology:

In order to isolate lignin degrading fungal strains from natural habitats, samples were collected from various sources ( rotten jute, rotten wood, saw dust ). Each substrate was inoculated separately with one known white rot fungi P. chrysosporium and other one is unknown organism. Control experiments were carried out under the same condition . After 14 days of fermentation ,enzymes were collected from the fermented substrates and from control one with water. Enzyme production was standard procedure. For the determination of lignolytic capability of the isolated strains,the fungi were grown on solid culture medium with poly – R , a sulphonated polymeric dye. CM Case, Xylanase and Pectinase activities were assayed by standard procedure modified by Mohiuddin.

Source of Information: Annual Technological Report, 2001, BJRI

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Enzymes production by solid state fermentation on different substrates with the fungs. Phenarochetea chrysosporium and one isolated unknown organisms were found . P. chrysosporium showed maximum xylanase on the substrate saw dust and the unknown isolated fungus showed xylanase on jute plant powder under same condition.

Knowledge Management: Report/Proceedings