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Isolation and Characterization of Microorganisms Producing Xylanase, Pectinase, Ligninase and Cellulase from Various Linno-cellulosic Agro-wastes Available in Different Parts of Bangladesh

A K M Rohul Kabir
SSO
Biochemistry Department, BJRI, Dhaka-1207

Dr.Isidore Gomes
PSO
Biochemistry Department, BJRI, Dhaka-1207

Abstract/ Executive Summary:

Mesophilic pectiolytic fungi were isolated from natural habitats. Iolated strains were screened for their pectinase activity in the selective media. The ability of isolates to improve the quality of jute cuttings will be tested gradually.

Keywords: Microorganism, Enzymes, Agro-waste

Location: Central station, BJRI,Dhaka

Start Date: 01-01-1998

End Date: 01-07-2001

Program Area: Resource Development and Management

Commodity: Jute

Objectives:

Improvement of the jute fibre quality by the application of pectinase, xylanase or ligninase enzymes produced by different groups of microorganisms.

Methodology:

In order to isolate mesophilic fungus sample of rotten wood, degraded jute etc were collected from different places. A small portion of the collected samples were put in sterile water and shaked well. A little of this liquor was inoculated in solid agar medium in petridish. Then the plates were incubated at 32⁰C for 3 – 7 days. The colonies were isolated and purified through subsequent subculture and plate cultures. Then the pure strain was isolated and maintained on PDA slant in refrigerator. The fungal strins were screened for their enzyme ( xylanase, pectinase) activities. Petriplates of respective media were used for the test of respective enzyme. The plates were inoculated with isolated fungalstrains to be screened for enzyme activity. After an appropriate incubation period, the activity was detected around the fungal colonies by the appearance of zone reveated by substrate hydrolysis around the colony followed by coloration or discoloration. Determination of pectinase activity, The plates were flooded with 1% cetyltrimeethyl ammonium bromide solution . Zones of hydrolysis were visible after 5 – 10 minutes of incubation at room temperature

Source of Information: Annual Technological Report,2001, BJRI

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

About 35 colonies were isolated for determination of pectinase activity. The isolates were marked as BJB – 1, BJB -2 and so on. Among them 5 isolates were selected as pectinolytic on the basis of their activity to grow on pectin medium. Rest of the isolates did not grow well indicating their lack of pectiolytic activity.

Knowledge Management: Report/Proceedings