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Genetic Modification of Fungal Strains by Gamma Radiation / Mutagenic Chemicals

Dr. S H Talukder
CSO incharge
Microbiology and Biochemistry Department,BJRI,Dhaka

Dr.Isidore Gomes
PSO
Microbiology and Biochemistry Department,BJRI,Dhaka

Afsana Marzina
SA
Microbiology and Biochemistry Department,BJRI,Dhaka

Abstract/ Executive Summary:

The thermophilic fungus RT – 9 was tried for its mutant strains. Several strains were successfully developed . But the enzyme activity of these mutant strains was not above the enzyme activity given by the original wild strain.

Keywords: Genetic, Mutants and Enzyme

Location: Central station, BJRI ,Dhaka

Start Date: 01-07-2000

End Date: 01-07-2001

Program Area: Knowledge Management

Non-commodity: Performance

Objectives:

Development of hyper enzyme producing fungal mutants from the known fungal strains

Methodology:

The fungus thermomyces lanoginasus ( RT – 9 ) is one of the potential fungi used for production of thermostable enzymes . In order to increase its enzyme producing capabilities the fungus was chosen for genetic modification by exposing it to gamma radiation at AERE,saver, Dhaka. The spore suspension of the fungus in sterile water was exposed to gamma radiaion for genetic mutation. Exposure timeto gamma radiation was fixed with the dose of 50% of the survival .One ml radiated spore suspension was spread were PDA plates and incubated at 50⁰C for 36 Hours. Individual fungal colony was transferred on the basis of their shape, size and colour. After successive transfer from one plate to another several stable mutants were developed in terms of colour and appearance and they were designated at RT1, RT2 RT3 and so on. These newly developed fungal mutants of RT -9 was tested for their enzyme producing capability on wheat bran medium.

Source of Information:

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

A number of stable mutants of fungus RT – 9 have developed by exposing to gamma radiation. But the enzyme productivity of newly developed mutants did not exceed the enzyme productivity of the original strain , RT – 9 S.

Knowledge Management: Report/Proceedings