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In Vitro and in Vivo Screening of Tea Clones at Nursery Level During Clonal Selection Stage for Nematode Susceptibility

Dr. Mainuddin Ahmed
Chief Scientific Officer
Department of Pest Management, BTRI, Srimangal, Moulvibazar

Mohammad Shameem Al Mamaun
Senior Scientific Officer
Entomology Division, BTRI, Srimangal, Moulvibazar

Shovon Kumar paul
Scientific Officer
Entomology Division, BTRI, Srimangal, Moulvibazar

Abstract/ Executive Summary:

The experiment was initiated to identify resistance/ susceptibility of a particular clone to nematodes at BTRI main farm. Pathogenic nematode culture was prepared in suitable media and keep stock samples. Pathogen free media or soil substrate by sterilization as control treatment was also prepared. The pathogenicity of the nematodes was observed regularly. Soil samples were collected from the experimental plot (primary bed) at nursery of released clones (T1-BT15, T2-BT16 and T3-BT17) as well as test clones (T4-A/8/8, T5-A/17/7 and T6-A/22/40) at monthly interval and analysed for nematode count at Entomology Laboratory through Baermann funnel method. Mortality percentages of different clones were also recorded.The partial result revealed that among the released clones BT16 and that was test clones A/17/7 showed some resistance against plant parasitic nematodes both in primary and secondary bed. The experiment is going on with other released and test clones by turns.

Keywords: Tea clones, Nursery, Nematode susceptibility

Location: BTRI Main Farm

Start Date: 01-01-2008

End Date: 31-12-2013

Program Area: Pest Management

Commodity: Tea

Objectives:

To identify resistance/ susceptibility of a particular clone to nematode

Methodology:

The experiment was initiated with Randomized Block Design (RBD) at BTRI nursery. Pathogenic nematode culture was prepared in suitable media and keep stock samples. Pathogen free media or soil substrate by sterilization as control treatment was also prepared. The pathogenicity of the nematodes was observed regularly. Soil samples were collected from the experimental plot (primary bed) at nursery of released clones (T1-BT15, T2-BT16 and T3-BT17) as well as test clones (T4-A/8/8, T5-A/17/7 and T6-A/22/40) at monthly interval and analysed for nematode count at Entomology Laboratory through Baermann funnel method. Mortality percentages of different clones were also recorded.

Source of Information: Annual Report 2012

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The partial result revealed that among the released clones BT16 and that was test clones A/17/7 showed some resistance against plant parasitic nematodes both in primary and secondary bed. The experiment is going on with other released and test clones by turns.

Knowledge Management: Report/Proceedings