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In Vitro Screening of Potato Germplasms Against Salinity

Dr. Farida Begum
Chief Scientific Officer
Tuber Crops Research centre, BARI, Joydebpur, Gazipur

A.U. Haque
Scientific Officer
Tuber Crops Research centre, BARIJoydebpur, Gazipur

B.C. Kundu
Principal Scientific Officer
Tuber Crops Research centre, BARIJoydebpur, Gazipur

M.M. Hossain
Tuber Crops Research centre, BARIJoydebpur, Gazipur

Abstract/ Executive Summary:

Single node explants and three genotypes of potato were grown for 30 days in a solid MS media containing different NaCl concentrations (0, 4, 8, 12, 16 and 20 dS-m). Salt tolerance was assessed by its effect on the different growth parameters of both root and shoot (length, number and fresh weight). Root growth is more affected than the shoot growth by salinity. Lower salinity has little or no effect on potato plantlet. At 20dS-m Diamant and MF II failed to grow. Among the three genotypes Cardinal performed better under salinity. Cardinal absorbed more water as compared to control as a result fresh weight increased and thus help cardinal to survive under stress condition by diluting the internal ion concentration.

Keywords: Screening, Potato germplasm, Salinity

Location: Gazipur

Start Date: 01-11-2011

End Date: 30-05-2012

Program Area: Variety and Species

Commodity: Potato

Objectives:

i) To find out the effect of different level of NaCl on in vitro growth of potato cultivars using single node cutting

ii) To evaluate salt tolerant cultivars effective for future use under salinity condition.

Methodology:

A laboratory experiment was conducted in Tissue Culture laboratory, TCRC, to study the salinity tolerance of potato. Single node cuttings of potato plantlet derived from sprout culture were used in this experiment. Plantlets of Cardinal, Diamant and MF II were grown in vitro condition. Plantlets were prepared with shoot apex and roots were discarded. Single nodes were separated and cultured in MS media having 3% sucrose, 0.8% agar with a range of NaCl (O, 4, 8, 12, 16 and 20dS-m) treatments. Eight replications of each genotypes at each salinity level were used and grown for 30 days. The growth in respect of days to root initiation, no. of roots, length of root and shoot, no. of leaves, fresh weight and dry weight of plantlets were measured to evaluate their salinity tolerance.

Source of Information: TCRC Annual Report-2011-12, BARI

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 1,50,000/=

Total Budget (Tk.) : 1,50,000/=

Achievement/Findings:

Cardinal perform better and can be included in the varietals improvement program to salt stress conditions. Cardinal absorbs more water as compared to control and as a result fresh weight is increased thus helping cardinal to survive under stress condition by diluting the internal ion concentration.

Knowledge Management: Report/Proceedings