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Effect of Rootstock and Method of Grafting on the Success and Growth of Sweet Orange (malta)

R. Chakma
Scientific Officer
Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh.

M. R. Amin
Professor
Department of Horticulture, Sher-e-Bangla Agriculture University, Dhaka.

M. A. J. Bhuyan
Director
Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh.

M. A. UIlah
Principal Scientific Officer
Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh.

Abstract/ Executive Summary:

The experiment was conducted at the nursery of Hill Agricultural Research Station, Khagrachari from August 2009 to February 2010 to find out the effect of rootstock and grafting method on the success and growth of sweet orange (Malta). The experiment consisted of two factors with four rootstocks (Rangpur lime, Rough lemon, Kalamanchi and Pummelo) and three grafting method (Cleft, Veneer and Splice grafting). The highest percentage of graft success (86.3%) was found in Rangpur lime (R₁), while the lowest (81.9%) in Kalamanchi (R₃). The highest percentage of graft survivability (77.3%) was recorded in Rough lemon (R₂) and Pummelo (R4) while the lowest (75.1 %) in Kalamannchi (R3). The highest percentage of graft success (91.1%) was found in Cleft grafting (G₁) and the lowest (76.7%) in Splice grafting (G₃). The highest percentage of graft survivability (83.0%) was found in Cleft grafting (G₁) and the lowest (68.8%) in Splice grafting (G₃). The highest percentage of graft success (94.2%) was observed in Rough lemon in Cleft grafting (R₂G₁), whereas the lowest (74.2%) was recorded in Rough lemon in Splice grafting (R₂G₃). The highest percentage of graft survivability (87%) was observed in Pummelo in Cleft grafting (R₄G_I) whereas the lowest (66.0%) was recorded in Rough lemon in Splice grafting (R₂G₃).

Keywords: Rootstock, Method, Growth, Success and Sweet Orange.

Location: Hill Agricultural Research Station, Khagrachari area.

Start Date: 00-08-2009

End Date: 00-02-2010

Program Area: Crop-Soil-Water Management

Commodity: Orange

Objectives:

To find out the appropriate rootstock, suitable grafting method, and success and survivability of grafting for Sweet orange.

Methodology:

The experiment was conducted at the nursery of Hill Agricultural Research Station, Khagrachari from August 2009 to February 2010. For the experiment, scions were collected from the plant of BARI Malta-I. Four rootstocks i.e. Rangpur lime, Rough lemon, Kalamanchi/Calamondin and Pummelo and three grafting methods i.e. Cleft, Veneer and Splice grafting were used in this experiment. The two factors experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. The grafting operation was done in between 14-16 August 2009. For each method, a total of 90 operations were made. The rootstock seedlings were raised in poly bags (8"x6"). The poly bags were filled with soil and cow dung in equal ratio. Proper care and management of the seedlings were taken to maintain their good health. Sraight, healthy, pest and disease free Scions with swollen terminal bud in bulging condition were used from non flowering shoots having fresh growth, dark green colour leaves. Leaves on the scion sticks were removed without damaging the buds and then grafted onto rootstocks. The grafts were watered regularly. Intercultural operations like weeding, pest and disease control etc. were done as and when required. Data on days to bud break, percent of graft success (30 days after grafting, DAG), leaf number of new growth, number of shoots per graft, survivability and stionic volume of canopy (N-S, E- W) at 180 DAG were recorded and analyzed statistically. The percentage of survival and canopy volume at 180 days after grafting were calculated by using the following formulae: = (Total number of graft done-Total number of died graft/Total number of graft done) x 100 Collected data for various characters were statistically analyzed using MST A T program. Mean for all the treatments was calculated and the analysis of variance for each of the characters was performed by F (variance ratio) test. Difference between treatments was evaluated by Duncan's Multiple Range Test(DMRT) test at 5% level of significance (Gomez and Gomez, 1984).

Source of Information: J Expt. Biosci. 4(1): 29-34, January, 2013, ISSN 2223-9626 (Online), ISSN 2077-3358 (Print)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It may be concluded that rough lemon was found to be more suitable as rootstock in cleft grafting for propagation of sweet orange having greater percent of graft success along with survivability.

Knowledge Management: Journal