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Isolation of Microbes from Different Natural Environmental Sources (orange Peel, Onion, Banana, Guava, Curd, Cucumber, Retting Effluent from Nagar Kanda Upazila, Faridpur and Monirampur Etc.) and Studies of Their Retting Properties

Dr. Firoza Akhtar
Principal Scientific Officer
Post harvest Processing Department, Fibre Quality Improvement Division, BJRI, Dhaka-1207.

M. Younus Ali
Senior Scientific Officer (cc)
Post harvest Processing Department, Fibre Quality Improvement Division, BJRI, Dhaka-1207.

Abstract/ Executive Summary:

The experiment was undertaken to isolate microbes (bacteria & fungi) from different natural sources. The experiment was carried out in the laboratory of Fibre Quality Improvement Division during the period of 2011-2012. First of all, different natural sources containing unknown microbes were collected and those were analyzed in a sterile condition for hunting newer active jute retting microbes for retting purpose within a short period. Ten different fungi were isolated and identified. Among them 5 fungi e.g. Aspergillus flavus, Aspergillus sp., Aspergillus niger, Botryodiplodia sp,and Penicillium sp. were good retter, three different fungi e.g. Trichoderma sp., Penicillium sp. III, Mucor sp. were moderate retter and the other fungi were non retter.

Keywords: Isolation, Microbes, Natural sources, Retting properties.

Location: Laboratory of Fibre Quality Improvement Division.

Start Date: 00-00-2011

End Date: 00-00-2012

Program Area: Pest Management

Commodity: Jute

Objectives:

i) To isolate microbes (bacteria & fungi) from different natural environmental sources

ii) To determine the retting properties of microbes on jute and allied fibre plants.

Methodology:

Different strains of microbes were collected from different natural and environmental sources such as orange peel, Onion, Banana, Guava, Curd, Cucumber, Retting effluent from Nagar kanda upazila, Faridpur, Jute research station Monirampur and Faridpur and grown in PDA, nutrient broth, peptone agar and other selective synthetic media at room temperature using standard microbiological methods and the microbes were then identified. The deshi and tossa jute varieties viz. CVL-1 and O-9897 were grown in different jute research stations and then green jute stems were brought to the Laboratory of Fibre Quality Improvement Division. The stems were cut into 8 inches pieces and the identified microorganisms were applied on the pieces of green jute stem and their retting properties were assessed.

Source of Information: Annual Report 2011-2012

Article Link (Online Journal):

Funding Source:

1. Government

Budget: Fifteen thousand only

Total Budget (Tk.) : 15000/-

Achievement/Findings:

Ten fungi were isolated from various sources such as orange peel, Onion, Banana, Guava, Curd, Cucumber, Retting effluent from Nagar kanda upazila, Faridpur, Jute research station Monirampur and Faridpur. Among the isolated fungi 5 fungi e.g. Aspergillus flavus, Aspergillus sp., Aspergillus niger, Botryodiplodia sp,and Penicillium sp. were good retter, three different fungi e.g. Trichoderma sp., Penicillium sp. III, Mucor sp. were moderate retter and the other fungi were non retter.

Knowledge Management: Report/Proceedings, Booklet/Leaflet