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Microtuber Production of Potato as Influenced by Bap and Sucrose

M.A. Hossain
Senior Scientific Officer
Tuber Crops Research centre, BARI, Joydebpur, Gazipur

A.U. Haque
Tuber Crops Research centre, BARI, Joydebpur, Gazipur

M. Hossain
Tuber Crops Research centre, BARI, Joydebpur, Gazipur

Abstract/ Executive Summary:

Virus free Diamant cultures were multiplied on MS medium containing 1.0 mg/l GA₃. When maximum mumber of shoots was produced cultures were shifted on microtuber induction Medium. In vitro tuberization media consisted of MS along with different concentrations of BAP and sucrose. It was observed that 90 g/l sucrose supplemented with 10.0 mg/l BAP in the mediums resulted in maximum tuber induction (16.33) and highest weight (9.21 g).

Keywords: Microtuber, Potato, BAP, Sucrose

Location: Gazipur

Start Date: 01-06-2008

End Date: 30-05-2009

Program Area: Variety and Species

Commodity: Potato

Objectives:

To determine the optimum level of BAP and sucrose for getting higher proportion of microtubers both in number and weight.

Methodology:

In vitro ready stocks of plantlets of potato variety, Diamant were used and the number of plantlets increased by subculturing via nodal cutting at 3 weeks interval. The multiplication medium contained mineral salts (Murashige and Skoog, 1962) and 0.1mg/l GA3, 0.01 mg/l NAA, 4 mg/l calcium pantathonate and 30g/l sucrose. The medium was solidified with 8 g/l agar. Temperature in the growth chamber was 22+20C with 16 hours photoperiod and light was supplied by fluorescent tubers at an intensity of 3000 lux. Eight stem segments (each with 3 nodes) of subcultured in vitro plantlets were cultured in flasks containing 40 ml microtuber induction medium, which was based on MS medium supplemented with four levels of BAP (0, 5, 10 and 15 mg/l) and eight levels sucrose (30, 40, 50, 60, 70, 80, 90 and 100 g/l) and incubated at 22+20C under dark condition. The experiment was laid out in a Completely Randomized design (CRD) with two factors having three replications. The induced microtubers were harvested aseptically after 60 days of incubation period. The collected data were analyzed statistically and the mean separation was done by DMRT.

Source of Information: TCRC Annual Report - 2008-2009

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 50,000/=

Total Budget (Tk.) : 50,000/=

Achievement/Findings:

Maximum number of tubers in number (16.33/flask) and weight (9.21 g/flask) were obtained when MS medium was supplemented with combination of 10.0 mg/l BAP and 90 g/l sucrose.

Knowledge Management: Report/Proceedings