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Performance of Sago and Tapioca Powder as an Alternative to Agar for in Vitro Shoot Regeneration of Potato

M.A. Hossain
Senior Scientific Officer
Tuber Crops Research centre, BARIJoydebpur, Gazipur

A.U. Haque
Tuber Crops Research centre, BARI, Joydebpur, Gazipur

A.T.M Tanjimul Islam
Tuber Crops Research centre, BARI, Joydebpur, Gazipur

M. Hossain
Tuber Crops Research centre, BARI, Joydebpur, Gazipur

Abstract/ Executive Summary:

The efficiency of in vitro shoot regeneration and microtuber production of potato from nodal explants was studied using agar and other solidifying agent’s tapioca and sago in Murashige & SKoog (MS) salt medium. For shoot regeneration. Agar was maintained at 0.8%, tapioca and sago were varied between 9-18% and 1.14% respectively. Tapiocak agar and sago significantly affected in vitro plant regeneration. After 32 days, shoot height and number of shoots respectively were 8.5 cm and 8.0 in agar, and 10.1 cm and 8.0 in 15% tapioca, 3.9 cm and 6.0 in 1% sago, respectively. The result showed the possibility of a successful use of tapioca, a cheaper solidifying substance, for in vitro culture of potato.

Keywords: Sago, Tapioca, Regeneration, Potato

Location: Gazipur

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Potato

Objectives:

To compare the efficacy of alternative cheaper solidifying agent (s) namely tapioca and sago to costly item agar for in vitro shoot regeneration using nodal explants of potato.

Methodology:

The shoot proliferation study was done using Diamant, obtained serially subcultured every four weeks using 10 mm long nodal plants having one bud and a leaf axil. The medium was prepared using MS salt dissolved in double distilled water and consisted of 3% sucrose. The pH was adjusted to 5.8 before boiling the medium. Agar was maintained at the standard concentration (8 g/l) and tapioca and sago was used form (9, 10, 11, 12, 14, 15, 17 and 18%) and (1.0, 1.2, 1.4%), respectively. Two nodal cuttings taken form four weeks old plantlets were cultured in 30 ml Ms medium supplemented with 0.25 mg/l GA₃, 0.03 mg/l NAA and 2.0 mg/l Ca- panthothenate. In case of tapioca and sago, the required concentrations (w/v) were calculated per volume of media to be dispensed in each culture vessel following the protocol developed by Nene and Sheila (1997). The sutures were incubated for 35-40 days at 24⁰C with 16 h photoperiod. The experiment was laid out as CRD and replicated three times.

Source of Information: TCRC Annual Report - 2008-2009, BARI

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 50,000/=

Total Budget (Tk.) : 50,000/=

Achievement/Findings:

Tapica has the possibility to use successful as a cheaper solidifying substance, for in vitro culture of potato.

Knowledge Management: Report/Proceedings