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Research Detail

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Dr. M Shamsher Ali
PSO
Biotechnology Division, BRRI, Gazipur

Objective of this study was to evaluate the production of double haploid from F1's through Anther Culture. Higher percentage of callus produced by IR6852-100-1-2/RDA (10.88%) and excellent regeneration was obtained from WEIY on 989/BG400-1 cross (50.00%). Successful F1's were selected for anther culture to develop desired variety within a short time.

  Double haploid, Anther culture, Variety development.
  Biotechnology Lab, BRRI
  01-07-2004
  30-06-2005
  Variety and Species
  Rice

To evaluate the production of double haploid from F1's through Anther Culture.

Anther culture is the technique used in complement to breeding for development of varieties with the desired traits in a shorted period of time by producing double haploid plants. Potential lines may be fixed through anther culture. Anthers of 12 F1s grown in T.Aman/2004 were plated in M1 media after 8 days incubation of boot. Four F1s responded to callus induction.

  BRRI. Proceedings of the BRRI Annual Internal Review for 2004-2005. Bangladesh Rice Research Institute (BRRI). Gazipur -1701.
  
Funding Source:
1.  Government Budget:  
  

Higher percentage of callus produced by IR6852-100-1-2/RDA (10.88%) and excellent regeneration was obtained from WEIY on 989/BG400-1 cross (50.00%). Five F1s out of 8 grown in Boro/05 produced callus. Higher percentage of callus produced by BR4848-54-4-1-4-9/BR41/BR29/Pokkali (2.89%) followed by BR802-78-2-2-1/BR40/BR802-78-2-2-1/Pokkali (2.62) and the highest regeneration was obtained from BR29/BR41/BR28?Basmoti370 (50.00%) followed by BR802-78-2-2-1/BR40/BR802-78-2-2-1/Pokkali (42.86%). Thirty eight albino and twenty green plants were regenerated from BR29/BR41/BR28/Basmoti370 and BR 802-78-2-2-1/BR40/BR802-2-2-1/Pakkali respectively.

  Report/Proceedings
  

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