Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effect of Banana Extract on Growth and Development of Protocorm Like Bodies in Dendrobium Sp. Orchid

M. Obaidul Islam
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh - 2202

Md. Serazul Islam
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh - 2202

MS Sabina Yasmin
Scientific Officer
RHRS, Akbewrpur, Moulovibazar, Shylhet

Md. Abu Saleh
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh - 2202

Abstract/ Executive Summary:

An experiment was carried out in Plant Tissue Culture Laboratory, Department of Crop Botany, Bangladesh Agricultural University, Mymensingh to investigate the effect of banana extract on micropropagation of Dendrobium sp. var. Sonia orchid through PLBs. The experiment was conducted during July 2012 to October 2013. Half-Murashige and Skoog (1/2MS) medium were used as basal medium and the medium was supplemented with banana extract at 12.5, 25, 50, 100, and 200 ml L^-1 with a control, where no banana extract was supplemented. The cultures were done in 100 ml conical flasks and maintain at 25°C with 30µ mol m^-2 S^-1 lighting provided by florescent tubes for 16 hours per day. Banana extract showed significant effect on growth and development of PLBs. Among the treatments, 100 ml L^-1 banana extract enhanced new PLBs regeneration from explanted PLBs and growth and development of PLBs. Present research indicated that nutrient requirement for PLBs multiplication and plantlets growth of Dendrobium orchid is quantitatively different in vitro. Finally, 100 ml L^-1 and 25 ml L^-1 of banana extract may be recommended as supplement into 1/2MS medium for PLBs multiplication and plantlet regeneration, respectively in vitro.

Keywords: Banana extract, Dendrobium orchid, Protocorm like bodies, Regeneration

Location: Plant Tissue Culture Laboratory, Department of Crop Botany, Bangladesh Agricultural University (BAU), Mymensingh

Start Date: 00-07-2012

End Date: 00-10-2013

Program Area: Crop-Soil-Water Management

Commodity: Orchid

Objectives:

To investigate the effect of BE as supplement into culture medium on regeneration of new PLB, growth of PLBs and PLB derived plantlets of Dendrobium sp. in vitro.

Methodology:

Protocorm like bodies (PLBs) of Dendrobium sp. var. Sonia developed earlier from meristem culture and has been maintained by subculturing bimonthly on fresh medium in the Plant Tissue Culture Laboratory, Department of Crop Botany, Bangladesh Agricultural University (BAU), Mymensingh, were used as plant materials. This experiment was conducted during July 2012 to October 2013 in the same laboratory. Several cultures of PLBs and PLBs derived plantlets were conducted in the experiment. To study the effect on growth and development of PLBs and plantlets 0, 25, 50, 100 and 200 ml L^-1 of banana extract were supplemented into culture medium. The experiment was laid out in a Completely Randomized Design (CRD) and each treatment was replicated five times. Murashige and Skoog medium was diluted to half strength (only macro elements) and was used as basal medium for PLB proliferation, plantlet initiation and the subsequent growth and development of initiated plantlets. Five stock solutions namely, macronutrients, micronutrients, Fe-EDTA, vitamins/organics and plant growth regulators were prepared prior to culture medium preparation. Stock solution of macro-nutrients was prepared 5 times higher than the concentration of medium used in experiment. Five times higher quantity of salt ingredients were taken in 1.0 L conical flask and dissolved in distilled water, serially by using a magnetic stirrer. Finally, distilled water was added up to the mark. Prepared solution was preserved in a refrigerator at 4°C for later use. Similarly, stock solution of micro nutrients (lOa times), Fe-EDTA (l00 times) and vitamins/organics (l00 times) were prepares and stored. The ripe banana (cv. Sagar) was peeled and cut into 1.0 em in size and 500 g of the diced fresh material was boiled for 30 minutes with 500 ml of distilled water and the hot supernatant was filtered through steel mesh (55 mm^-1) and adjusted up to 500 ml by adding distilled water. The data on average number of newly regenerated PLBs per flask, number of PLBs, weight per PLB, number of plantlet initiation, weight of plantlets, number of leaf and root per plantlets etc. were recorded after culture. The means were calculated and analyses of variance for all the characters were performed by F test. The significance of the difference among the pair of means was evaluated by Duncan's Multiple Range Test (DMRT) at 5% level of significance using MST AT -C computer programmed.

Source of Information: The Agriculturists 13(1): 101-108 (2015), ISSN 2304-7321 (Online), ISSN 1729-5211 (Print)

Article Link (Online Journal):

Funding Source:

1. Bangladesh Agricultural Research Council

Budget: 900000

Total Budget (Tk.) :

Achievement/Findings:

Organic extracts is a complex substance containing different levels of carbohydrate, amino acids, proteins, vitamins, phenoloc acids and many alkaloid compounds. Any of these component(s) could be responsible for promoting growth and development of the cultures. Which ingredient or ingredients were supportive for growth and development of PLBs or plantlets in vitro are to be clarified by further detail study. However, the present study indicates that nutritional requirement is different in PLBs and PLB-derived plantlets. Finally, it might be concluded that banana extract could help in building an organic medium for in vitro production of Dendrobium orchids.

Knowledge Management: Journal