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Isolation and Identification of Plant Parasitic Nematodes Associated with Commercial Crops

F. Elahi
Scientific Officer
Plant Pathology Division, BARI, Gazipur

Dr. M.I. Faruk
Senior Scientific Officer
Plant Pathology Division, BARI, Gazipur

Abstract/ Executive Summary:

A survey was carried out to find out the nematodes association with commercial crops available throughout the country during the period October, 2012 to February, 2013.Twelve crops sample were collected from different places from 7 districts of Bangladesh and tested in the laboratory. From the collected samples Pratylenchus sp. (Lesion nematodes), Meloidogyne spp. (Root knot nematode), Tylenchus sp., Hoplolaimus sp., Helicotylenchus sp., Tylenchorhynchus sp., Zygotylenchus sp. were found.

Keywords: ISOLATION, IDENTIFICATION, NEMATODES

Location: Joypurhat, Khagrachari, Sherpur, Jamalpur, Gazipur, Tangail, and Barisal

Start Date:

End Date:

Program Area: Pest Management

Commodity: Diseases and insects

Objectives:

To identify the available nematodes association with commercial crops in some districts of Bangladesh.

Methodology:

Plants were uprooted and root system together with the clinging soil and surrounded soil were collected in polythene bags from Joypurhat, Khagrachari, Sherpur, Jamalpur, Gazipur, Tangail, and Barisal districts. Suspected soil and plant samples were brought into laboratory and soil was incubated for 72 hours in Baermann funnel and some roots were stained with lactophenol-acid fuchsin for the detection of internal parasites. Determination of species was made on living and dead specimen under stereo and compound microscope. Different structures of plant parasitic nematodes like types of style, esophagus, median bulb, reproductive organ and tails etc. were observed noted and identified the different genera of plant parasitic nematodes with the help of pictorial key to genera of Plant parasitic nematodes (Mai and Lyon 1975).

Source of Information: Annual Report, Plant Pathology Division 2012-13

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

A total of 7 different genera of plant parasitic nematodes were identified from 7 districts of Bangladesh which are belonging to the order Tylenchida.

Knowledge Management: Report/Proceedings