Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Pcr-based Method to Detect Pathogenic Microorganisms in Exportable Shrimp and Fish Products of Bangladesh

Dr. Md. Shaheed Reza
Associate Professor
Department of Fisheries Technology, Bangladesh Agricultural University, Mymenginsh-2202, Bangladesh

Abstract/ Executive Summary:

Since Polymerase Chain Reaction (PCR) represents a rapid procedure with both high sensitivity and specificity for immediate detection and identification of pathogenic bacteria from different food materials to ensure food safety, attempts were made to utilize PCR-based method to detect pathogenic Salmonella spp. and Escherichia coli in seafood principally from shrimp and prawn products ready to be exported. Initially, PCR conditions were standardize d using purified bacterial DNA. First Salmonella spp. and E. coli bacteria were collected from sewage water, cultured in selective media then transferred to broth culture, and then a rapid boil method was tested to isolate DNA from bacterial cells which was used for subsequent PCR analysis. Out of six pair of primers designed for two species of bacteria (3 pair each for Salmonella and E. coli), primerpair5´-GTGAAATTATCGCCACGTTCGGGCAA-3´and5´-TCATCGCACCGTCAAAGGAACC-3´ amplifying a DNA fragment of 284 bp of invA gene in Salmonella and primer pair 5´-GCTGGGCAGCAAACTGATAACTCTC-3´ and 5´ CATCAAGC TGTTTGTTCGTCCGCCG 3´ amplifying 750 bp of afa gene in E. coli were successful. Primers used in the PCR assay were highly specific for individual organisms and did not show cross-reactivity with heterologous organisms. In conclusion, the PCR-based technique provides a sensitive and specific method for detection of Salmonella spp. and E. coli.

Keywords: PCR, Technology, Shrimp, Pathogen bacteria.

Location: Mymenginsh

Start Date: 01-04-2010

End Date: 31-12-2012

Program Area: Pest Management

Commodity: Shrimp

Objectives:

To detect pathogenic microorganisms in exportable shrimp and fish products of Bangladesh and to utilize PCR-based method to detect pathogenic Salmonella spp. and Escherichia coli in seafood principally from shrimp and prawn products ready to be exported

Methodology:

Initially, PCR conditions were standardize dsing purified bacterial DNA. First Salmonella spp. and E. coli bacteria were collected from sewage water, cultured in selective media then transferred to broth culture, and then a rapid boil method was tested to isolate DNA from bacterial cells which was used for subsequent PCR analysis.

Source of Information: BAU Research proceedings, Vol.23, 2013, PP.84

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The PCR assay were highly specific for individual organisms and did not show cross-reactivity with heterologous organisms. In conclusion the PCR-based technique provides a sensitive and specific method for detection of Salmonella spp. and E. coli.

Knowledge Management: Report/Proceedings