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Epidemiological Investigation and Molecular Detection of Brucella in Cattle and Goats in Bangladesh

Dr. Md. Ariful Islam
Associate Professor
Department of Microbiology & Hygiene, Bangladesh Agricultural University Mymensingh-2202, Bangladesh,

Abstract/ Executive Summary:

The present study was conducted for epidemiological investigation and molecular detection of Brucella spp. in cattle and goats. Blood samples were collected from cattle (n = 150) and goat (n = 113) on farms, slaughter houses, veterinary clinics in Tangail, Mymensingh and Sherpur districts of Bangladesh. Information of animal’s age, sex, breeding practice, history of abortion was recorded during collection of samples. Aborted tissues such as: fetal abomasal content, lymph nodes, spleen and lungs were collected from 3 cases of abortion in cattle. The serum samples were screened for Brucella specific antibody response by rose Bengal plate test (RBPT). RBPT positive blood samples and aborted tissues were cultured on blood and brucella agar media for isolation and identification of Brucella. The overall seroprevalence of brucellosis was 15.33% in cattle. Prevalence of brucellosis in cattle was 22% in Tangail, 5% in Mymensingh and 16.66% in Sherpur. Prevalence of Brucella was higher in female (19.54%) as compared to male (8.21%). Higher prevalence of brucellosis was recorded in old cattle above four years of age (18%). Cattle with the history of abortion, retained placenta and repeat breeder showed 10%, 4% and 2.85% prevalence of brucellosis, respectively. The overall prevalence of brucellosis recorded in goats was 6.19%. She goats showed higher prevalence of brucellosis (5%) as compared to male goats (2%). Goats over 2 years of age showed the highest prevalence (4%) than other ages. Bacteriological screening of blood samples of cattle and goats and aborted tissues of cows did not yield bacterial growth characteristics of Brucella. Data of this study suggest that brucellosis is endemic in cattle and goats in the study area. More survey data are required in order to assess the actual prevalence of brucellosis in cattle and goats.

Keywords: Cattle, Goats, Brucellosis, Molecular detection

Location: BAU, Mymensingh

Start Date: 00-07-2011

End Date: 00-06-2012

Program Area: Animal Health and Management

Commodity: Cattle

Objectives:

To investigation epidemiological and molecular detection of Brucella spp. in cattle and goats

Methodology:

The experiment conducted in July 2011 to June 2012 at Bangladesh Agricultural University to investigation epidemiological and molecular detection of Brucella spp. in cattle and goats. Brucellosis is an economically important disease of domesticated livestock. The present study was conducted for epidemiological investigation and molecular detection of Brucella spp. in cattle and goats. Blood samples were collected from cattle (n = 150) and goat (n = 113) on farms, slaughter houses, veterinary clinics in Tangail, Mymensingh and Sherpur districts of Bangladesh. Information of animal’s age, sex, breeding practice, history of abortion was recorded during collection of samples. Aborted tissues such as: fetal abomasal content, lymph nodes, spleen and lungs were collected from 3 cases of abortion in cattle. The serum samples were screened for Brucella specific antibody response by rose Bengal plate test (RBPT). RBPT positive blood samples and aborted tissues were cultured on blood and brucella agar media for isolation and identification of Brucella.

Source of Information: BAU Research proceedings, Vol.23, 2013, PP.2

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The overall result eas seroprevalence of brucellosis was 15.33% in cattle. Prevalence of brucellosis in cattle was 22% in Tangail, 5% in Mymensingh and 16.66% in Sherpur. Prevalence of Brucella was higher in female (19.54%) as compared to male (8.21%). Higher prevalence of brucellosis was recorded in old cattle above four years of age (18%). Cattle with the history of abortion, retained placenta and repeat breeder showed 10%, 4% and 2.85% prevalence of brucellosis, respectively. The overall prevalence of brucellosis recorded in goats was 6.19%. She goats showed higher prevalence of brucellosis (5%) as compared to male goats (2%). Goats over 2 years of age showed the highest prevalence (4%) than other ages. Bacteriological screening of blood samples of cattle and goats and aborted tissues of cows did not yield bacterial growth characteristics of Brucella. Data of this study suggest that brucellosis is endemic in cattle and goats in the study area. More survey data are required in order to assess the actual prevalence of brucellosis in cattle and goats.

Knowledge Management: Report/Proceedings