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Molecular Epidemiologic Study on Rotaviruses Causing Severe Diarrhea in Man, Animal and Poultry of Bangladesh

Dr. Md. Mahbub Alam
Professor
Department of Medicine Bangladesh Agricultural University, Mymensingh-2202Bangladesh

Abstract/ Executive Summary:

Rotavirus is distributed worldwide as an enteric pathogen in man, animal and poultry. The highest rate of HRV infection was detected in the winter season (18.82%) and in the month of January (23.08%) and no detection was in August and September. The occurrence of rotavirus infection in human patients of 7-12 months age groups were relatively high (14.13%) while lowest (3.17%) occurrence was in above 5 years old. The prevalence of rotavirus diarrhea was slightly higher in male (11.83%) than female (10.62%) patients. No calves, sheep and goat fecal sample was found positive for rotavirus on PAGE-SS technique. During the study period, 429 fecal samples were tested of chicks and only three was found positive for rotavirus infection (0.70%; 3/429) on PAGE-SS technique. The positive sample was detected from 10-20 days layer chicks in the winter season. The migration patterns of detected positive strains were not similar in polyacrylamide gel electrophoresis and their migration speed was different types. Five electropherotypes were determined among 60 human rotaviruses. All the electropherotypes were long pattern. The genome migration of avian rotavirus type was distinct from human types.

Keywords: Diarrhea, Rotavirus, Animal, Poultry

Location: Department of Medicine, Bangladesh Agricultural University, Mymensingh

Start Date: 00-07-2010

End Date: 00-06-2012

Program Area: Animal Health and Management

Commodity: Poultry, Sheep, Goat, Cattle

Objectives:

The study on rotaviruses causing severe diarrhea in man, animal and poultry of Bangladesh

Methodology:

Rotavirus is distributed worldwide as an enteric pathogen in man, animal and poultry. In this study the epidemiology of rotavirus infection in human, calves, sheep, goats and poultry were studied. A total of 1197 fecal samples were collected from July 2010 to June 2012. These 1197 samples were consisted of 535 human, 148 calves, 82 sheep, 10 goats and 429 poultry and examined by Polyacrylamide Gel Electrophoresis and Silver Staining (PAGE-SS) technique for the detection of presence of rotavirus dsRNA.

Source of Information: BAU Research proceedings, Vol.23, 2013, PP.11 and Annual workshop on BAU research Progress: 2010-2011; BAURES, 02-03 April 2012, BAU, Mymensingh, 02-03-2012

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The study found that highest rate of HRV infection was detected in the winter season (18.82%) and in the month of January (23.08%) and no detection was in August and September. The occurrence of rotavirus infection in human patients of 7-12 months age groups were relatively high (14.13%) while lowest (3.17%) occurrence was in above 5 years old. The prevalence of rotavirus diarrhea was slightly higher in male (11.83%) than female (10.62%) patients. No calves, sheep and goat fecal sample was found positive for rotavirus on PAGE-SS technique. During the study period, 429 fecal samples were tested of chicks and only three was found positive for rotavirus infection (0.70%; 3/429) on PAGE-SS technique. The positive sample was detected from 10-20 days layer chicks in the winter season. The migration patterns of detected positive strains were not similar in polyacrylamide gel electrophoresis and their migration speed was different types. Five electropherotypes were determined among 60 human rotaviruses. All the electropherotypes were long pattern. The genome migration of avian rotavirus type was distinct from human types. In the present study, it was not investigated that bacteria, parasites or any other pathogens responsible for causing diarrhea or not.

Knowledge Management: Report/Proceedings