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Assessing Nutational Effect on Xylanolytic Fungi for Xylanase Production

Selina Akhter
Scientific Officer
Biochemistry Department, BJRI,Dhaka.

Dr. Mst. Aleya nasreen
Principle Cientific Officer
Biochemistry Department, BJRI,Dhaka

Md.Mahabub Ali
Scientific Officer
Biochemistry Department, BJRI,Dhaka

Abstract/ Executive Summary:

N. crassa secretes xylanase to the culture supernatant ,since activity was detected in mycelium cultures of the fungus. The cultures grown on xylan yielding detectable xylanase activities. But xylanase activity of wild type was found to be lower than that of UV ray induced mutated N.crassa . Wild N. crassa showed lowest xylanase activity . Mutant – 5 showed higest (1.66 IU) and mutant -4 showed second highest (1.184 IU ) activities. The reason is UV mutation may cause some genetic change in the gene that is responsible for xylanase production.

Keywords: Assessing, Xylanase, Mutants

Location: Central station,BJRI

Start Date: 01-01-2011

End Date: 01-07-2012

Program Area: Crop-Soil-Water Management

Commodity: Jute

Objectives:

To investigate the activities of enzymes, wild and mutants of N. crassa were grown on VM liquid medium separately in which the glucose was replaced by carboxy methyl cellulose at 0.5 % concentration.

Methodology:

A wild type and seven mutants of Neurospora crassa was collected from Dhaka University. To investigate the activities of enzymes, wild and mutants of N. crassa were grown on VM liquid medium separately in which the glucose was replaced by carboxy methyl cellulose at 0.5 % concentration. The fungi were incubated at 30 degree centigrade for 3 days in shake culture. The mycelia and other non soluble material in the culture filtrates were separated by centrifugation at 15,000rpm for 5 min. The clear supernatants were used for enzyme assays. For this purpose, standard curves of reducing sugar for CMCase were prepared. Reducing sugars were determined by measuring the absorbance at 540 nm. Hydrolytic activity was calculated by measuring the amount of reducing sugars released from CMC. The amount of reducing sugar was measured by the DNS method using glucose as standards for CMCase activities.

Source of Information: BJRI, Dhaka

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

N,crassa secretes xylanase to the gulture supernatant ,since activity was detected to mycelium cultures of the fungus. But this xylanase activity of wild type was found to be lower than that of UV ray induced mutant N, crassa . The enzymatic assays of this study indicate the presence of a hydrolytic system in N.crassa ,which was able to grow in media containing xylane as the sole carbon source.

Knowledge Management: Report/Proceedings