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Bulk Segregant Analysis of F2 Population Obtained from the Cross Between Var, O-4 and Incisifolius

Md. Samiul Haque
Senior Scientific Officer, Physiology Department, Agronomy Division
Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207.

Hasina Khan
Professor
Biochemistry Department, Dhaka University.

Abstract/ Executive Summary:

Bulk segregant analysis is a rapid mapping strategy suitable of mono genic qualitative traits. DNA samples isolated from 92F2 (obtained from cross between var. 0-4 and incisifilius) plants are being analyzed with 46 RAPD (randomly amplified polymorphic DNA) primary. DNA of 10 F2 plants which have glossy leaves and 10 which have matte leaves were bulked. Similarly DNA of 10 F2 plants which have entire leaf morphology and 10 which have deep serration were bulked. The resulting DNA bulks are used together with the parental samples in trying to obtain molecular markers (RAPD) linked to the glossy and deep serration trait respectively. This work is in its preliminary stage but there is a possibility to link molecular markers to the glossy trait and leaf shape traits of jute. Although these traits are not commercially important, the identification of one marker will lead to the linkage of another and eventually help us to create a genetic map of various traits of jute.

Keywords: Segregant, Population, Analysis, Incisifolius

Location: Green house, BJRI and Biochemistry Laboratory, Dhaka University

Start Date: 04-04-2002

End Date: 04-12-2002

Program Area: Variety and Species

Commodity: Jute

Objectives:

To assess the the molecular marker for different traits.

Methodology:

Source of Information: nnual Research Report on jute: 2002-2003, Bangladesh Jute Research Institute.

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 50,000

Total Budget (Tk.) : 50,000

Achievement/Findings:

The resulting DNA bulks are used together with the parental samples in trying to obtain molecular markers (RAPD) linked to the glossy and deep serration trait respectively. This work is in its preliminary stage but there is a possibility to link molecular markers to the glossy trait and leaf shape traits of jute. Although these traits are not commercially important, the identification of one marker will lead to the linkage of another and eventually help us to create a genetic map of various traits of jute.

Knowledge Management: Report/Proceedings