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Rejuvenation of Degenerating Sugarcane Germplasm Through in Vitro Culture

M R Alam
Scientific Officer
Breeding Division, BSRI, Ishurdi, Pabna

Sk. A Mannan
Chief Scientific Officer
Breeding Division, BSRI,Ishurdi, Pabna

S M N Nahar
Chief Scientific Officer
Head,Breeding Division, BSRI, Ishurdi, Pabna

H M Tarique
Scientific Officer
Breeding Division, BSRI, Ishurdi, Pabna

Abstract/ Executive Summary:

This experiment was conducted to regenerate the degenerating varieties/clones of sugarcane in the tissue culture laboratory of Breeding Division at BSRI, Ishurdi, Pabna during the cropping season 2010-2011. Different aged somatic or meristemetic tissues were cultured in MS (Murashige and Skoog, 1962) medium and modified MS medium supplemented with growth regulators to produce in vitro plantlets. Under this programme, degenerating sugarcane germplasms were rejuvenated using low concentrated 2,4-D (0.25mg/l) and GA3 separately in the in vitro culture system. In total 187 in vitro seedlings were developed from 6 degenerating genotypes of GP 76-3626, 82-1089, Isd 5-55, Co 1158, PR 1000 and L5, and planted in the field. After evaluation 8 rejuvenated lines from those genotypes were selected and planted in 4m ´ 4m stage for further evaluation.

Keywords: Rejuvenation, Degeneration, Sugarcane

Location: BSRI, Ishurdi, Pabna

Start Date: 01-07-2007

End Date:

Program Area: Variety and Species

Commodity: Sugarcane

Objectives:

To regenerate the degenerating varieties of sugarcane
To get benefit from the degenerating varieties of sugarcane

Methodology:

Degenerating sugarcane germplasm of BSRI were included in this programme. The experiment were carried out in the tissue culture laboratory of Breeding Division at BSRI, Ishurdi, Pabna. Different aged somatic or meristemetic tissues as explants were collected from field grown degenerating plants at different times. General aseptic techniques concerning in vitro plant tissue and organ culture were followed. The explant were cultured in MS (Murashige and Skoog, 1962) medium and modified MS medium supplemented with growth regulators to produce in vitro plantlets. Later on, plantlets were potted in glasshouse prior to acclimatization in natural conditions. Finally, newly developed plantlets were transplanted in the field and data will be recorded for comparison with the donor plant. TREATMENTS: T1 : Co 1158 T2 : Co 604 T3 : CP 71-1089 T4 : China T5 : CP 72-1218 T6 : CPI 228-80 T7 : VMC 86-550.

Source of Information: Annual Research Programme 2010-2011 & Annual Report 2011-12

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 3,90,000.00

Total Budget (Tk.) : 3,90,000.00

Achievement/Findings:

6 degenerating genotypes of GP 76-3626, 82-1089, Isd 5-55, Co 1158, PR 1000 and L5 were rejuvenated and planted in the field.

Knowledge Management: Report/Proceedings