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Development of Novel Rice Varieties by the Application of Molecular Breeding to Address Climate Change Issues and Food Security in Bangladesh

Dr. Lutful Hassan
Professor
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh,

Abstract/ Executive Summary:

Three F₂ rice breeding populations were screened to evaluate the use of molecular markers to salinity tolerance at seedling stage with electrical conductivity (EC) of 12 dSm-1. Twenty Simple Sequence Repeat (SSR) and two Expressed Sequence Tag (EST) markers from 49.6 to 87.1 cM position of chromosome 1 segment were used. Among them only 7 markers found polymorphic among the two parents for population 1, four markers for population 2 and five for population 3. From single marker analysis it was observed in population 1, RM8094 was found to be strongly associated with salinity tolerance (P<0.001) while other four markers RM1287, RM3412, RM493 and CP03970 significant at P<0.05. All of the markers were found in one linkage group flanking the SalTol region on chromosome 1 in F₃ population. RM8094 was found to be strongly associated with salinity tolerance with significant (P<0.0001) using single marker analysis. A total of 115 genotypes were used for allelic diversity study of microsatellite markers linked to a major SalTol QTL in chromosome 1 segment. Among the seven markers, the highest number of allele (15) was found in RM8094 followed by RM1287, RM3412 and RM493 (10), RM140 (8), CP03970 (5) and CP6224 (3) produced the lowest number of alleles. The polymorphic information content (PIC) values for seven markers from 115 genotypes was found from 0.54 to 0.89. The highest PIC value (0.89) was also found in RM8094 locus followed by RM493 and RM3412 (0.81), RM1287 and RM140 (0.77). So RM8094 would be used as the most suitable marker for marker assisted selection breeding program for SalTol QTL in chromosome 1 from the cross of widely diverse genotypes followed by RM3412 and RM493.

Keywords: Salinity tolerance, Rice, Climate change, Food security

Location: Mymensingh

Start Date: 00-00-2011

End Date: 00-00-2012

Program Area: Variety and Species

Commodity: Rice

Objectives:

To develop novel rice varieties by the application of molecular breeding.

Methodology:

Three F₂ rice breeding populations were screened in the year 2011-2012 to evaluate the use of molecular markers to salinity tolerance at seedling stage with electrical conductivity (EC) of 12 dSm-1. Twenty Simple Sequence Repeat (SSR) and two Expressed Sequence Tag (EST) markers from 49.6 to 87.1 cM position of chromosome 1 segment were used.

Source of Information: BAU Research proceedings, Vol.23, 2013, PP.37

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Among them only 7 markers found polymorphic among the two parents for population 1, four markers for population 2 and five for population 3. From single marker analysis it was observed in population 1, RM8094 was found to be strongly associated with salinity tolerance (P<0.001) while other four markers RM1287, RM3412, RM493 and CP03970 significant at P<0.05. All of the markers were found in one linkage group flanking the SalTol region on chromosome 1 in F₃ population. RM8094 was found to be strongly associated with salinity tolerance with significant (P<0.0001) using single marker analysis. A total of 115 genotypes were used for allelic diversity study of microsatellite markers linked to a major SalTol QTL in chromosome 1 segment. Among the seven markers, the highest number of allele (15) was found in RM8094 followed by RM1287, RM3412 and RM493 (10), RM140 (8), CP03970 (5) and CP6224 (3) produced the lowest number of alleles. The polymorphic information content (PIC) values for seven markers from 115 genotypes was found from 0.54 to 0.89. The highest PIC value (0.89) was also found in RM8094 locus followed by RM493 and RM3412 (0.81), RM1287 and RM140 (0.77). So RM8094 would be used as the most suitable marker for marker assisted selection breeding program for SalTol QTL in chromosome 1 from the cross of widely diverse genotypes followed by RM3412 and RM493.

Knowledge Management: Report/Proceedings