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Improvement of Garlic Through in Vitro Regeneration and Chromosomal Manipulation

Dr. Md. Asraful Haque
Associate Professor
Department of Genetics and Plant Breeding Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

Three garlic cultivars G-1, G-2 and G-3 were used in the present study. Different concentrations and combinations of growth regulators were used in MS medium to observe the callus induction ability of different explants. Considering all the cultivars collectively the range of callus induction was observed as to 0 to 85%. The highest callusing was obtained at the concentration of 2, 4-D (2.0mgl–1) + BAP (2.0mgl–1) in MS medium in most of the cases. For the regeneration, MS medium supplemented with 1.0mgl–1 BAP and 2.0mgl–1 NAA was recorded best for the regenerating ability of the three cultivars. The regenerated plantlets were then successfully transferred to the pot and after proper hardening the survival rate was satisfactory. For cytological study, chromosome preparation was made from the root tip by haematoxyline method. In all the three garlic cultivar 2n = 16 chromosomes was found. Though chromosome number of three garlic cultivars was the same but some variations in respect of length and arm ratio of the individual chromosome was found and it is also assumed that karyotypic differences as occurring in this three garlic cultivar could be due to structural changes of chromosomes.

Keywords: Garlic, Regeneration, Chromosomes

Location: Department of Genetics and Plant Breeding, BAU, Mymensingh

Start Date: 00-00-2011

End Date: 00-00-2012

Program Area: Variety and Species

Commodity: Garlic

Objectives:

To establish an efficient protocol for regeneration of garlic via callus culture and direct regeneration from root tips and cytological observation of local garlic cultivars from root tip.

Methodology:

The study was carried out to establish an efficient protocol for regeneration of garlic via callus culture and direct regeneration from root tips and cytological observation of local garlic cultivars from root tip. Three garlic cultivars G-1, G-2 and G-3 were used in the present study. Healthy root tips and basal disc of the three garlic cultivars were included for tissue culture studies.

Source of Information: BAU Research proceedings, Vol.23, 2013, PP.39-40

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The research found that in G-1, 5 metacentric, 2 submetacentric and 1 subtelocentric chromosomes, while considering the total length and arm ratio in every cases. In G-2, 7 metacentric and 1 submetacentric chromosome and in G-3, 6 metacentric and 2 submetacentric chromosome was found. Though chromosome number of three garlic cultivars was the same but some variations in respect of length and arm ratio of the individual chromosome was found and it is also assumed that karyotypic differences as occurring in this three garlic cultivar could be due to structural changes of chromosomes.

Knowledge Management: Report/Proceedings