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Extraction, Chromatographic Isolation of Bishkatali (polygonum Hydropiper L.), Neem (azadirachta Indica) and Study of the Effect of Biopesticide Compounds

Mr. Kartik Chandra Saha
Professor
Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh 2202Bangladesh

Abstract/ Executive Summary:

Extraction and chromatographic separation of Bishkatali (Polygonum hydropiper L.) plants powder were carried out in the Post Graduate Research Laboratory, Department of Agricultural Chemistry, BAU, Mymensingh during July 2011 to June 2012. Bishkatali weed is very familiar medicinal plant in this subcontinent and also used as an acaricide as well as pesticided. Selective extraction of the plant powder was performed by using solvents like hexane, ethanol and water respectively. The different extracting crude materials identified by thin layer chromatography (TLC) and the spots were visualized in the iodine chamber. The components were marked from their traveled corresponding Rf-values. Firstly the ethanolic crude material was washed out with 10% brine solution and then separated by applying the method of column chromatography using mixed solvent benzene : ethyl acetate (60 : 40 v/v). The two compounds A1 and A2 showed single spot under iodine vapour having Rf-values 0.882 and 0.796 of corresponding solvent system hexane : benzene (60 : 40 v/v) and benzene : ethyl acetate (9 : 1 v/v) respectively.

Keywords: Extraction, Chromatographic isolation, Biopesticide, Neem Biskatali

Location: Mymensingh

Start Date: 01-07-2011

End Date: 30-06-2012

Program Area: Pest Management

Commodity: Insecticide

Objectives:

1) To develop extraction, chromatographic isolation of Bishkatali (Polygonum hydropiper L.), Neem (Azadirachta indica) and study of the effect of biopesticide compounds

2) To separate of Bishkatali (Polygonum hydropiper L.) plants powder

Methodology:

Source of Information: BAU Research proceedings, Vol.23, 2013, PP.45

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The components were marked from their traveled corresponding Rf-values. Firstly the ethanolic crude material was washed out with 10% brine solution and then separated by applying the method of column chromatography using mixed solvent benzene : ethyl acetate (60 : 40 v/v). The two compounds A1 and A2 showed single spot under iodine vapour having Rf-values 0.882 and 0.796 of corresponding solvent system hexane : benzene (60 : 40 v/v) and benzene : ethyl acetate (9 : 1 v/v) respectively.

Knowledge Management: Report/Proceedings