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Research Detail

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Mrs. Kohinoor Begum
Senior Scientific Officer
Physiology and Sugar Chemistry Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Nadira Islam
Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Dr. Md. Amzad Hossain
Chief Scientific Officer
Head, Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Md. Shamsul Arefin
Scientific Officer
Physiology and Sugar Chemistry Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Dr. Md. Ibrahim Talukder
Chief Scientific Officer
Head, Pathology Division, BSRI, Ishurdi, Pabna

Dr. Md. Abdus Samad Miah
Director General
BSRI, Ishurdi, Pabna

This experiment was conducted to develop red rot, salt and drought tolerance in sugarcane varieties or clones. Somaclones developed from sugarcane varieties Isd 16, Isd 20, Isd 28 and Isd 33 were used for field evaluation at the Bangladesh Sugarcane Research Institute (BSRI) farm, Ishurdi, Pabna during 2010-2011 cropping season. One hundred and eleven somaclones of four varieties were used in the experiment. Each of somaclones was planted at spacing of 1m × 0.45m. Planting materials showed significant variation on tiller number, millable cane and cane yield. Fourteen somaclones were inoculated with red rot pathogen. Among the fourteen somaclones none of somaclones showed resistance reaction against red rot pathogen. All of inoculated somaclones showed susceptibility against red rot pathogen. One somaclone of variety Isd 20 showed moderately susceptibility against red rot pathogen. Six somaclones of varieties Isd 16 (1 somaclone), Isd 20 (4 somaclones), Isd 28 (1 somaclone) showed susceptibility against red rot pathogen while rest of red rot inoculated 7 somaclones shoed highly susceptible to red rot pathogen. Among donor sugarcane varieties used for somaclone development Isd 20 is tolerance to red rot reaction, however somaclones of this variety showed moderately susceptibility to red rot reaction. This is perhaps due to change of genetic material during somaclones development which indicates the possibility of production of variable somaclones against red rot pathogen and may be used to produce and selection of red rot tolerance somaclones for red rot tolerant sugarcane varieties.

  Sugarcane, Somaclone
  BSRI Lab, Pot and Terokhada-Khulna and Barind Tract Rajshahi
  01-07-2002
  
  Variety and Species
  Sugarcane

a. To study the effects of red rot toxin, NaCl, hydroxyl prolin and polyethylene glycol concentrations on different in vitro stages. b. To study the effects of red rot, salinity and drought on regenerated plants under ex vitro conditions. c. To develop red rot, salt and drought tolerance in sugarcane varieties or clones.

a. BSRI released varieties prone to biotic and abiotic stress were used as experimental materials. b. Somaclones from defective varieties were produced through callus culture. c. For callus initiation explants were inoculated on MS + 2,4-D(3mg/l) + 10% coconut water. d. Callus were sub-cultured on the medium containing red rot fungal toxin isolated using standard procedure (5.0, 7.5, 10.0, 12.0, and 15.0%), NaCl (50,100, 150, 200 and 250 mM), HYP (0.5, 1.0, 1.5, 2.0 and 2.5 mM) and PEG (5.0, 7.5, 10.0, 12.5 and 15.0%). On every concentration the callus were maintained for 2-3 weeks. e. The tolerant callus were tested for retention of tolerance by sub-culture first on media without selecting agents, followed by selecting agents containing media. f. Shoot were regenerated on shooting media (MS+2mg/l BAP + 1mg/l Kn) with and without selecting agents. g. For root initiation regenerated shoots were sub-cultured on the medium MS + NAA (5mg/l) with and without selecting agents. h. After hardening, plants were tested under induced red rot, salinity and drought condition under pot and field conditions.

  Annual Research Programme 2010-2011 & Annual Report 2011-12
  
Funding Source:
1.  Government Budget:  2,09,989.00
   2,09,989.00

Red rot inoculated one somaclone of variety Isd 20 showed moderately susceptibility against red rot pathogen indicated the possibility of production of variable somaclones against red rot pathogen which may be used to produce and selection of red rot tolerance somaclones for variety development against red rot disease.

  Report/Proceedings
  

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