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In Vitro Preservation of Sugarcane Germplasm

M R Alam
Scientific Officer
Breeding Division, BSRI, Ishurdi, Pabna

Sk. A Mannan
Chief Scientific Officer
Breeding Division, BSRI, Ishurdi, Pabna

S M N Nahar
Chief Scientific Officer
Breeding Division, BSRI, Ishurdi, Pabna

K M R Karim
Scientific Officer
Breeding Division, BSRI, Ishurdi, Pabna

B P Podder
Chief Scientific Officer
Head,Breeding Division, BSRI, Ishurdi, Pabna

Abstract/ Executive Summary:

This experiment was conducted to preserve the genetic materials in in vitro condition in the germplasm laboratory of BSRI, Ishurdi, Pabna during the cropping season 2010-2011. For in vitro short-term preservation the Mannitol (5%) and Sucrose (5%) supplemented MS medium showed effective performance to preserve explants under refrigerator condition upto12 months. After preservation of 12 months, the preserved explants of Isd 17 were cultured in shooting medium and showed resumption of growth. From preserved explants of Isd 17 in vitro seedlings were planted, evaluated and selected. The selected line of Isd 17 PG (2) was planted in G3 generation at 4m × 4m stage. Besides, the shoot tip explants of CP 50-70, Col 66 and I 103-96 were survived for 10 months in in vitro preserve condition, and they showed resumption of growth when cultured in shooting medium

Keywords: In Vitro Preservation, Sugarcane

Location: BSRI, Ishurdi, Pabna

Start Date: 01-07-2001

End Date:

Program Area: Variety and Species

Commodity: Sugarcane

Objectives:

I. To preserve the genetic materials in in vitro condition II. To select the suitable media composition for in vitro preservation

Methodology:

Methodology in this study were carried out in two phases as following: (1) SOURCE, COLLECTION, STERILIZATION AND PREPARATION OF EXPLANT The explant, which were used as the preserved material, were collected from actively field grown plant. After surface sterilization about 1.0 cm long sprout cutting shoot tips were excised as explant for culture in the medium. (2) STORAGE CONDITION AND PROCEDURE In this study for short to medium-term preservation of explant, MS medium containing three protectants at different concentrations and combinations were used. The cultures were maintained both in refrigerator (10-12C) under dark and culture room conditions. After preservation period, explant were regenerated in MS medium supplemented with different hormones. TREATMENTS 1) MS +Mannitol + Sucrose 2) MS + Glycerol + Sucrose 3) MS +Mannitol + Glycerol + Sucrose

Source of Information: Annual Research Programme 2010-2011 & Annual Report 2011-12

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 3,91,200.00

Total Budget (Tk.) : 3,91,200.00

Achievement/Findings:

For in vitro short-term preservation, Mannitol (5%) and Sucrose (5%). supplemented MS medium showed effective performance to preserve explants under refrigerator condition upto12 months.

Knowledge Management: Report/Proceedings