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Nodulation, Nitrogen Fixation and Nitrogenase Activity of Two Mungbean Genotypes Differing in Salt Tolerance

Md. Abdul Aziz
SSO
Agronomy Division, BARI, Gazipur

M.A.Karim
Professor
BSMRAU, Salna, Gazipur

Abstract/ Executive Summary:

Salt tolerance of mungbean gennotypes BM 01 and BM 21 was assessed for nodulation, nitrogen fixation and nitrogenase activity at 0, 50, 75 and 100 mM NaCl solutionin in pot culture. Salt stress lead to a significant reduction in number of nodules per plant but the reduction in BM 21 was higher than that in BM 01. Nitrogenase activity significantly decreased with increasing salinity levels and it was 38% in BM 01 while that was 59% in BM 21 at 100 mM NaCl. The nodule dry matter reduction occurred across a wider range (58% in BM 01 and 83 % BM 21) while the range of variations in nitrogenase activity was rather narrow (38% in BM 01 and 59 % BM 21). It might be concluded that higher nodulation and nitrogen fixation in BM 01 than BM 21 contributed to the high salt tolerance of the former genotype than BM 21.

Keywords: Salt tolerance, Mungbean, Nodulation, Nitrogen fixation, Nitrogenase activity

Location: BSMRAU, Salna, Gazipur

Start Date: 15-05-1999

End Date: 20-06-1999

Program Area: Variety and Species

Commodity: Mungbean

Objectives:

To clarify the reasons for high salt tolerance of BM 01 compared to BM 21 in terms of nodulation, nitrogen fixation and nitrogenase activity.

Methodology:

Two mungbean genotypes, BM 01 (tolerant one) and BM 21 (susceptible one), were sown in earthen pots in the vinyl house. Each pot was filled with 12 kg air-dried soil. Compost (1/4th of the soil volume) and 0.27 0.28 0.20 g urea, TSP and MP per pot were incorporated uniformly into the soil. The pots were irrigated with tap water until the seedlings were well established. NaCl solutions were applied every other day at an increment of 12.5 mM until the respective final concentrations, 50, 75 and 100 mM were attained for each treatment. The treatment solution was applied with irrigation water until harvest. Plants in the control group were irrigated with tap water. Plant samples were collected from the pot at flowering stage. Three pots were randomly selected from each salinity levels of each genotype. Lastly six plants were selected randomly from three pots. Plants were carefully uprooted under flow of tap water so that no nodules were left in the soil. Nitrogenase activity of the plants was assessed by gas chromatography. The nodules from the roots of each plant were separately collected and weighed. The root, stem, leaves and nodules were oven–dried at 70^oC to a constant weight.

Source of Information: Ph.D Thesis, BSMRAU, Salna, Gazipur

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 30000

Total Budget (Tk.) : 30000

Achievement/Findings:

The nodule dry matter reduction occurred across a wider range (58% in BM 01 and 83 % BM 21) while the range of variations in nitrogenase activity was rather narrow (38% in BM 01 and 59 % BM 21). It might be concluded that higher nodulation and nitrogen fixation in BM 01 than BM 21 contributed to the high salt tolerance of the former genotype than BM 21.

Knowledge Management: Report/Proceedings